Effect of Wnt6 on human dental papilla cells in vitro

Journal of Endodontics
Chenglin WangLing Ye

Abstract

The Wnt signaling pathway plays an important role in tissue development by acting on proliferation, differentiation, and cell fate decisions. Because the role of Wnt6 in tooth development was still unknown, the purpose of this study was to investigate the role of Wnt6 in tooth morphogenesis and dental tissue mineralization by elucidating its effect on human dental papilla cells (hDPCs) in vitro. Human dental papilla cells were enzymatically separated from tooth germs. Recombinant adenovirus encoding full-length Wnt6 cDNA was constructed to overexpress Wnt6, and the biologic effects of Wnt6 on hDPCs were investigated. Wnt6-transduced changes in hDPC proliferation were examined by means of a 5-bromodeoxyuridine (BrdU) incorporation assay and cell cycle analysis. Wnt6-transduced changes in hDPC differentiation were investigated by evaluating alkaline phosphatase (ALPase) activity, by a mineralization assay, and analysis of mineralization-related gene expression including ALP, type I collagen (Col I), osteonectin (ON), osteopontin (OPN), bone sialoprotein (BSP), and dentin matrix protein-1 (DMP-1). Wnt6 overexpression had no significant effect on the proliferation of hDPCs by BrdU incorporation assay and flow cytometric analysis. W...Continue Reading

Citations

Oct 11, 2011·Connective Tissue Research·Kevin A Tompkins
Jun 22, 2011·Journal of Endodontics·Heng LinZhi Chen
May 9, 2015·Scientific Reports·Bo GaoLiwei Zheng
Aug 23, 2011·Biochemical and Biophysical Research Communications·Tak-Heun KimEui-Sic Cho
Mar 30, 2017·PloS One·Xueyang LiaoLing Ye
Apr 29, 2016·American Journal of Physiology. Renal Physiology·Hayley BeatonJohn Crean
Sep 29, 2020·Frontiers in Cell and Developmental Biology·Yuta ChibaSatoshi Fukumoto
Jan 12, 2021·Frontiers in Cell and Developmental Biology·Ming WeiHui Zhao
Jul 20, 2021·Frontiers in Cell and Developmental Biology·Ran ZhangGuan Yang

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