PMID: 8611613Jan 12, 1996Paper

Effect of X31 influenza virus fusion on phosphatidylserine asymmetry in erythrocytes

Biochimica Et Biophysica Acta
C H Pak, R Blumenthal

Abstract

Influenza virus fusion is mediated by its fusion protein, hemagglutinin (HA). HA undergoes a low pH dependent conformational change that results in insertion into the cell membrane bilayer, formation of a fusion pore, and merging of membrane lipids and establishment of cytoplasmic continuity. Erythrocytes, which can serve as targets of influenza virus fusion, display an asymmetric transbilayer arrangement of their phospholipids. The effect of influenza virus fusion on erythrocyte phosphatidylserine asymmetry was determined. Influenza virus were bound to erythrocytes containing the fluorescent membrane probe NBD-PS in the inner leaflet. Induction of fusion by exposure to a low pH environment resulted in movement of PS to the outer leaflet of the cell as well as hemolysis. Insertion of the fusion protein into erythrocytes and subsequent fusion can be distinguished from hemolysis by examining the interaction of a soluble form of HA (BHA) with cells and by monitoring viral fusion at low temperatures. No hemolysis was observed under either condition. BHA binding and insertion into cells did not affect the asymmetry of PS. Incubation of influenza virus fusion at pH 5, 0 degrees C resulted in complete fusion but no outward movement of...Continue Reading

References

Nov 6, 1992·Science·J M White
Dec 1, 1992·The Journal of Cell Biology·J R Monck, J M Fernandez
Nov 13, 1991·Biochimica Et Biophysica Acta·A J Schroit, R F Zwaal
May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·A E SpruceW Almers
Dec 10, 1990·FEBS Letters·J BentzD Alford
Jul 1, 1985·The Journal of Cell Biology·S J DoxseyJ White
Jun 12, 1987·Biochimica Et Biophysica Acta·P H Van der SchaftL L Van Deenen
Mar 1, 1972·Nature: New Biology·M S Bretscher
Aug 2, 1972·Nature: New Biology·C M Brand, J J Skehel
Jun 1, 1981·The Journal of Cell Biology·J WhiteA Helenius
Apr 1, 1984·The Journal of General Virology·R W RuigrokJ E Mellema
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·S B SatoS Ohnishi
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M Seigneuret, P F Devaux
Jul 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·T MaedaS Ohnishi
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J J SkehelD C Wiley
Jun 1, 1981·The Journal of Clinical Investigation·B LubinL L Van Deenen
Jan 1, 1993·Annual Review of Biophysics and Biomolecular Structure·J ZimmerbergL V Chernomordik

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