Effect of xylitol on salivary β-glucosidase in humans

European Journal of Oral Sciences
Lucimari Teixeira EssenfelderMaria L B Magalhães

Abstract

Dental biofilm - in which a diverse set of microorganisms are embedded in a complex polysaccharide matrix that adheres to oral components - is one of the most complex microbial communities in the human body. As biofilm formation is related to oral infections, such as caries and periodontal diseases, strategies for biofilm control are crucial for maintaining oral health. Xylitol, a synthetic sugar used as a sucrose substitute, has been shown to reduce biofilm formation. However, its precise mechanism of action on biofilm reduction has so far not been elucidated. Previous studies demonstrate that bacterial β-glucosidase action is crucial for biofilm formation. Here, we investigated the correlation between salivary β-glucosidase activity and dental plaque occurrence. We found a positive correlation between enzymatic activity and the presence of dental biofilm. We observed that xylitol inhibits β-glucosidase in human saliva. Kinetic studies also confirmed that xylitol acts as a mixed type inhibitor of salivary β-glucosidase. Based on our data, we suggest that xylitol impairs oral biofilm formation by the inhibition of bacterial β-glucosidase, which is essential for biofilm formation in the oral cavity.

References

Jan 1, 1975·Annual Review of Microbiology·R J Gibbons, J V Houte
May 1, 1989·Journal of Dental Research·R J Gibbons
Nov 1, 1985·Journal of Periodontal Research·J J ZambonJ Slots
Nov 1, 1966·Archives of Biochemistry and Biophysics·M V Kelemen, W J Whelan
Jan 1, 1982·Infection·J van Houte
Nov 1, 1983·Journal of Periodontal Research·M Nakamura, J Slots
Sep 20, 1993·Annals of the New York Academy of Sciences·M Navazesh
Dec 19, 2002·Critical Reviews in Biotechnology·Yukti BhatiaV S Bisaria
Nov 8, 2005·Journal of Clinical Microbiology·Jørn A AasFloyd E Dewhirst
Jan 30, 2008·Microbial Ecology·Anna M RomaníUrsula Obst
Sep 25, 2009·Current Microbiology·Eva M Söderling, Aija-Maaria Hietala-Lenkkeri
Aug 3, 2010·Nature Reviews. Microbiology·Hans-Curt Flemming, Jost Wingender
Nov 26, 2014·Clinical, Cosmetic and Investigational Dentistry·Prathibha Anand NayakVishal Khandelwal
Jun 7, 2015·Applied and Environmental Microbiology·Taichi InuiA Bryan Hanley
Jun 28, 2015·Archives of Biochemistry and Biophysics·Paulo H E WeissMaria L B Magalhães
Jul 18, 2015·Microbiology Spectrum·Dominique H LimoliDaniel J Wozniak
Mar 3, 2017·Journal of Natural Science, Biology, and Medicine·Chandrashekar JanakiramJoe Joseph
Apr 28, 2017·Archives of Oral Biology·Fathilah Abdul RazakMd Yusoff Musa
Sep 26, 2017·Nature Reviews. Microbiology·Hyun KooLuanne Hall-Stoodley
Dec 23, 2017·Journal of Molecular Graphics & Modelling·Sumith Yd, Shalabh C Maroo

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Citations

Jul 9, 2020·Applied Microbiology and Biotechnology·Asma Gasmi BenahmedGeir Bjørklund
Mar 19, 2021·Biochemistry and Biophysics Reports·Lucimari Teixeira EssenfelderMaria de Lourdes Borba Magalhães

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