Effect of zeta potential of cationic liposomes containing cationic cholesterol derivatives on gene transfection

FEBS Letters
K TakeuchiM Nakanishi

Abstract

Cationic liposomes are known to be useful tools for gene transfection. However, the relation between transfection efficiency and physicochemical properties of liposomes has not been well understood. Here, we synthesized eight cationic derivatives of cholesterol which contain a tertiary amino head group with a different spacer arm. Transfection of plasmid pSV2CAT DNA into cells was done by cationic liposomes made of a mixture of dioleoylphosphatidylethanolamine (DOPE) and each cationic cholesterol derivative. At the same time we measured zeta potential of cationic liposomes by laser Doppler spectroscopy. The present results indicated that zeta potentials of cationic liposomes were well related to transfection activity of pSV2CAT DNA. This suggested that zeta potential of cationic liposomes is one of important factors which control gene transfection.

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Citations

Jan 28, 2004·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yoshikazu InohMamoru Nakanishi
Aug 1, 1998·Journal of Controlled Release : Official Journal of the Controlled Release Society·R J MumperA P Rolland
Jun 15, 2000·Bioorganic & Medicinal Chemistry Letters·T RenD Liu
Apr 20, 2002·Bioorganic & Medicinal Chemistry Letters·Seiji HasegawaMamoru Nakanishi
Jul 31, 2009·Chemical Communications : Chem Comm·Santanu Bhattacharya, Avinash Bajaj
May 4, 2001·Anti-cancer Drugs·A G Schatzlein
Jun 2, 2007·Biological & Pharmaceutical Bulletin·Naohide HirashimaMamoru Nakanishi
Jun 7, 2006·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Mamoru Nakanishi
Nov 16, 2001·Biochemical and Biophysical Research Communications·Y InohM Nakanishi
Sep 2, 2003·Journal of Pharmaceutical Sciences·Brian A LoboC Russell Middaugh
Mar 7, 2015·Biological & Pharmaceutical Bulletin·Yoshiyuki HattoriEtsuo Yonemochi
Jan 3, 2013·International Journal of Pharmaceutics·Yoshiyuki HattoriYoshie Maitani
Nov 6, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Daniele PezzoliGabriele Candiani
Aug 3, 2010·International Journal of Pharmaceutics·Yoshikazu InohMamoru Nakanishi
Feb 28, 2009·Nanomedicine : Nanotechnology, Biology, and Medicine·Bizhan Malaekeh-NikoueiMohammad Ramezani
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