Effect of zwitterionic surfactants on the separation of proteins by capillary electrophoresis

Electrophoresis
B Y Gong, J W Ho

Abstract

The effect of zwitterionic surfactants on capillary electrophoretic separation of proteins was investigated using an uncoated silica capillary column and different buffers containing zwitterionic surfactants. The effects of N-alkyl-N,N-dimethyl ammonio-1-propane-sulfonates on the electrophoretic mobility of three different proteins, namely albumin, lysozyme and myoglobin, were examined. The addition of N-alkyl-N,N-dimethylammonio-1-propane sulfonate in phosphate saline buffer is important in minimizing protein-capillary wall interactions, and facilitated an efficient electrophoretic mobility of myoglobin and lysozyme. The separation efficiency of proteins also depends on the injection pressure for the control of migration time and the peak sharpness. The electrophoretic conditions were applied to evaluate the separation of lysozyme, myoglobin and albumin. The zwitterion surfactants can form a dynamic coating on the capillary surface, thereby reducing the number of adsorption sites to which proteins may adsorb.

References

Jan 1, 1991·Analytical Chemistry·M J GordonR N Zare
Jun 1, 1991·Analytical Chemistry·J K Towns, F E Regnier
Sep 7, 1990·Journal of Chromatography·J K Towns, F E Regnier
Sep 7, 1967·Biochemical and Biophysical Research Communications·A L ShapiroJ V Maizel

❮ Previous
Next ❯

Citations

Jul 20, 2000·Clinica Chimica Acta; International Journal of Clinical Chemistry·T MiuraM Kaku
Sep 19, 2003·Journal of Chromatography. a·Yuanzhong YangMilton T W Hearn
Aug 27, 1998·Journal of Chromatography. a·S R BeanG L Lookhart
Jan 1, 2008·Journal of Chromatography. a·Charles A LucyMakedonka D Gulcev
Nov 8, 2005·Electrophoresis·Amy M MacDonaldCharles A Lucy
Feb 28, 2002·Analytical Chemistry·Jennifer M CunliffeCharles A Lucy

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.