PMID: 11312255Apr 20, 2001Paper

Effective antigen presentation by dendritic cells is NF-kappaB dependent: coordinate regulation of MHC, co-stimulatory molecules and cytokines

International Immunology
S YoshimuraMarc Feldmann

Abstract

Antigen presentation is a key rate-limiting step in the immune response. Dendritic cells (DC) are the most potent antigen-presenting cells for naive T cells, due to their high expression of MHC and co-stimulatory molecules, but little is known about the biochemical pathways that regulate this function. We here demonstrate that monocyte-derived mature DC can be infected with adenovirus at high efficiency (>95%) and that this procedure can be used to dissect out which pathways are essential for inducing DC antigen presentation to naive T cells. Using adenoviral transfer of the endogenous inhibitor of NF-kappaB, IkappaBalpha, we show that DC antigen presentation is NF-kappaB dependent. The mechanism for this is that NF-kappaB is essential for three aspects of antigen-presenting function: blocking NF-kappaB coordinately down-regulates HLA class II, co-stimulatory molecules like CD80, CD86 and CD40, and immuno-stimulatory cytokines like IL-12 and tumor necrosis factor-alpha. In contrast adhesion molecules are up-regulated after infection with the adenovirus transferring IkappaBalpha, indicating that NF-kappaB also regulates the duration of T cell-DC interaction. These results establish NF-kappaB as an effective target for blocking D...Continue Reading

References

Jan 1, 1991·Annual Review of Immunology·R M Steinman
Apr 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·P S BrownW R Benjamin
Dec 1, 1988·Current Opinion in Immunology·D R Katz
Dec 1, 1980·The Journal of Experimental Medicine·G H SunshineM Feldmann
Jun 1, 1995·Molecular Biotechnology·F L Graham, L Prevec
Feb 1, 1995·Immunobiology·M J StaquetD Schmitt
Jan 1, 1994·Annual Review of Immunology·P Matzinger
Jan 1, 1993·The British Journal of Nutrition·I EngelbertsW A Buurman
Apr 1, 1993·Immunology and Cell Biology·K J StaceyD A Hume
Mar 19, 1996·Proceedings of the National Academy of Sciences of the United States of America·L J Zhou, T F Tedder
May 1, 1996·Current Biology : CB·C A JanewayR Medzhitov
Nov 1, 1996·Science·D J Van AntwerpI M Verma
Apr 1, 1998·Nature·J Banchereau, R M Steinman
Jul 8, 1998·Proceedings of the National Academy of Sciences of the United States of America·B FoxwellM Feldmann
Feb 19, 2000·Current Opinion in Immunology·S D ReidL Adorini

❮ Previous
Next ❯

Citations

Sep 1, 2009·Nature Chemical Biology·Zhonghua YanRuma Banerjee
Mar 11, 2006·Cell Death and Differentiation·V BotteroI M Verma
Oct 25, 2002·AIDS Research and Human Retroviruses·D MessmerM Pope
Aug 19, 2007·International Immunology·Matthew E HandleyBenjamin M Chain
Mar 2, 2012·International Immunology·Prachi VilekarVibhudutta Awasthi
Feb 25, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Paiman GhafooriSharmila Masli
Nov 11, 2005·American Journal of Therapeutics·Nick Giannoukakis, Massimo Trucco
Oct 24, 2012·Current Opinion in Allergy and Clinical Immunology·Rana S Bonds, Terumi Midoro-Horiuti
Jun 18, 2005·Acta Pharmacologica Sinica·Jun GuoCarlos R Becerra
Aug 19, 2005·Molecular and Cellular Biology·Pedro J CejasKelvin P Lee
Jun 30, 2007·American Journal of Respiratory Cell and Molecular Biology·Tibor Z VeresArmin Braun
May 19, 2005·Arthritis Research & Therapy·Bernhard M KirschMarcus D Säemann
Sep 15, 2006·Proceedings of the National Academy of Sciences of the United States of America·E AndreakosM Feldmann
Aug 24, 2005·Proceedings of the National Academy of Sciences of the United States of America·Qiutang LiInder M Verma
Jan 15, 2014·Cellular and Molecular Life Sciences : CMLS·Chiara GaspariniGiorgio Zauli
May 28, 2014·Materials Science & Engineering. C, Materials for Biological Applications·Rongrong ZhuShilong Wang
Jun 22, 2010·Expert Opinion on Therapeutic Targets·Dipender Gill, Peng H Tan
Jul 30, 2005·Expert Opinion on Biological Therapy·Wilson S Meng, Lisa H Butterfield
Feb 1, 2008·Expert Opinion on Drug Discovery·Jignesh Unadkat, Maryam Feili-Hariri
Mar 1, 2012·Human Immunology·Chiara GaspariniGiorgio Zauli
Sep 29, 2011·Toxicology and Applied Pharmacology·Ryohei Miyata, Stephan F van Eeden
Dec 16, 2010·Advanced Drug Delivery Reviews·Feng Li, Ram I Mahato
Jul 17, 2010·International Immunopharmacology·Jee Youn KimSang-Bae Han
Jan 22, 2010·Medical Hypotheses·Mark F McCartyFrancisco Contreras
Apr 2, 2009·International Immunopharmacology·Sun Young Park, Younghee Kim
Sep 23, 2008·Free Radical Biology & Medicine·Martha M SklavosJon D Piganelli
Mar 4, 2008·The Journal of Allergy and Clinical Immunology·Colin de HaarRaymond Pieters
Jul 28, 2005·International Journal of Experimental Pathology·Gabriele PollaraDavid R Katz
Apr 27, 2004·Periodontology 2000·Christopher W Cutler, Ravi Jotwani

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.