Effective delivery of the anti-mycobacterial peptide NZX in mesoporous silica nanoparticles

PloS One
Erik TenlandGabriela Godaly

Abstract

Intracellular delivery of antimicrobial agents by nanoparticles, such as mesoporous silica particles (MSPs), offers an interesting strategy to treat intracellular infections. In tuberculosis (TB), Mycobacterium tuberculosis avoids components of the immune system by residing primarily inside alveolar macrophages, which are the desired target for TB therapy. We have previously identified a peptide, called NZX, capable of inhibiting both clinical and multi-drug resistant strains of M. tuberculosis at therapeutic concentrations. In this study we analysed the potential of MSPs containing NZX for the treatment of tuberculosis. The MSPs released functional NZX gradually into simulated lung fluid and the peptide filled MSPs were easily taken up by primary macrophages. In an intracellular infection model, the peptide containing particles showed increased mycobacterial killing compared to free peptide. The therapeutic potential of peptide containing MSPs was investigated in a murine infection model, showing that MSPs preserved the effect to eliminate M. tuberculosis in vivo. In this study we found that loading the antimicrobial peptide NZX into MSPs increased the inhibition of intracellular mycobacteria in primary macrophages and preserv...Continue Reading

References

Jun 8, 1999·Annual Review of Immunology·A Aderem, D M Underhill
Aug 24, 1999·Infection and Immunity·V A SnewinD B Young
Feb 21, 2002·Journal of Controlled Release : Official Journal of the Controlled Release Society·Fakhrul AhsanAna I Torres Suarez
Sep 2, 2003·Nature Reviews. Immunology·Tomas Ganz
Feb 12, 2009·ACS Nano·Jessica M RosenholmMika Lindén
Jun 16, 2009·Nature Materials·Andre E NelMike Thompson
Jun 2, 2011·Molecular Therapy : the Journal of the American Society of Gene Therapy·Veronika MamaevaCecilia Sahlgren
Jan 5, 2012·Chemical Society Reviews·Zongxi LiJeffrey I Zink
Aug 28, 2012·Advanced Drug Delivery Reviews·Veronika MamaevaMika Lindén
Sep 18, 2012·Cell Host & Microbe·Constance J MartinSamuel M Behar
Dec 6, 2012·Nature Nanotechnology·Marco P MonopoliKenneth A Dawson
Nov 16, 2013·Nature Chemical Biology·Ashley L HilchieRobert E W Hancock
Aug 12, 2014·Protein and Peptide Letters·Ashaben PatelAshim K Mitra
Jan 15, 2016·Current Biology : CB·Ling-Juan Zhang, Richard L Gallo
Feb 27, 2016·Molecular Therapy : the Journal of the American Society of Gene Therapy·Veronika MamaevaCecilia Sahlgren
Mar 22, 2016·Mini Reviews in Medicinal Chemistry·Luigi PasquaCatia Morelli
May 14, 2016·Journal of Colloid and Interface Science·Katharina BraunMartin Malmsten
Jul 13, 2016·Expert Opinion on Drug Delivery·Rafael R CastilloMaría Vallet-Regí
Jul 12, 2017·Frontiers in Cellular and Infection Microbiology·Sadaf KalsumMaria Lerm

❮ Previous
Next ❯

Citations

Jan 1, 2020·Current Protein & Peptide Science·Sk Abdul Mohid, Anirban Bhunia
Mar 21, 2020·Daru : Journal of Faculty of Pharmacy, Tehran University of Medical Sciences·Maria Hassan KianiGul Shahnaz
May 18, 2020·Drug Delivery and Translational Research·Bushra NabiJaved Ali
May 13, 2020·Pharmaceutics·Rafael R CastilloMaría Vallet-Regí
Aug 8, 2020·Frontiers in Chemistry·Vanitha SelvarajanPui Lai Rachel Ee
Feb 20, 2021·Scientific Reports·Komal Umashankar RaoGabriela Godaly
Mar 18, 2021·Pharmaceutical Development and Technology·Afaf H Al-NadafNouf N Mahmoud
Nov 2, 2020·Colloids and Surfaces. B, Biointerfaces·Sandra Montalvo-QuirósJose L Luque-Garcia
Jun 7, 2021·Advanced Drug Delivery Reviews·Cheng WangJiang Xia
Jun 12, 2021·Colloids and Surfaces. B, Biointerfaces·Huma FatimaSunil Kumar Khare
Jul 9, 2021·International Journal of Nanomedicine·Rafael R Castillo, María Vallet-Regí
Sep 1, 2021·ACS Biomaterials Science & Engineering·Chee Wei AngMark A T Blaskovich
Sep 3, 2021·Advanced Drug Delivery Reviews·Alba García-FernándezRamón Martínez-Máñez
Nov 7, 2021·BMC Complementary Medicine and Therapies·Chang SunCunren Meng

❮ Previous
Next ❯

Methods Mentioned

BETA
dynamic light scattering
flow cytometry
electron microscopy
Confocal microscopy

Software Mentioned

ImageXpress
Graph Prism

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.