Effective enhancement of short-chain-length-medium-chain-length polyhydroxyalkanoate copolymer production by coexpression of genetically engineered 3-ketoacyl-acyl-carrier-protein synthase III (fabH) and polyhydroxyalkanoate synthesis genes

Biomacromolecules
Christopher T NomuraY Doi

Abstract

Polyhydroxyalkanoates (PHAs) are biodegradable polyesters that have a wide variety of physical properties dependent on the lengths of the pendant groups of the monomer units in the polymer. PHAs composed of mostly short-chain-length (SCL) monomers are often stiff and brittle, whereas PHAs composed of mostly medium-chain-length (MCL) monomers are elastomeric in nature. SCL-MCL PHA copolymers can have properties between the two states, dependent on the ratio of SCL and MCL monomers in the copolymer. It is desirable to elucidate new and low cost ways to produce PHA composed of mostly SCL monomer units with a small mol % of MCL monomers from renewable resources, since this type of SCL-MCL PHA copolymer has superior qualities compared to SCL homopolymer. To address this issue, we have created strains of recombinant E. coli capable of producing beta-ketothiolase (PhbA) and acetoacetyl-CoA synthase (PhbB) from Ralstonia eutropha, genetically engineered 3-ketoacyl-ACP synthase III (FabH) from Escherichia coli, and genetically engineered PHA synthases (PhaC) from Pseudomonas sp. 61-3 to enhance the production of SCL-MCL PHA copolymers from glucose. The cumulative effect of having two monomer-supplying pathways and genetically engineered...Continue Reading

References

Feb 1, 1997·Applied Microbiology and Biotechnology·S KusakaY Doi

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Citations

Nov 24, 2006·Applied Microbiology and Biotechnology·Christopher T Nomura, Seiichi Taguchi
Dec 11, 2007·Doklady. Biochemistry and Biophysics·A N BoyandinT G Volova
Jan 11, 2014·Biotechnology for Biofuels·Helge Jans Janßen, Alexander Steinbüchel
Jun 12, 2013·Bioscience, Biotechnology, and Biochemistry·Chitwadee PhithakrotchanakoonSutipa Tanapongpipat
Jan 18, 2012·Journal of Bioscience and Bioengineering·Ryan C TappelChristopher T Nomura
Dec 7, 2011·Journal of Bioscience and Bioengineering·Alexander P Mueller, Christopher T Nomura
Feb 23, 2010·Tetrahedron Letters·Umar AkbarDouglas S Clark
Nov 28, 2013·Journal of Applied Microbiology·T T MendonçaL F Silva
Nov 14, 2006·Bioresource Technology·Rui LiQingsheng Qi
Apr 14, 2016·Journal of Bioscience and Bioengineering·Lucia Salamanca-CardonaChristopher T Nomura
Feb 24, 2015·International Journal of Biological Macromolecules·Jaykishan H DhangdhariyaSandhya Mishra
Aug 8, 2008·Mikrobiologiia·A N BoiandinT G Volova
Dec 5, 2019·Bioengineering·Dario PuppiFederica Chiellini
Mar 7, 2021·Bioengineering·Alberto GiubiliniGilberto Siqueira
Dec 11, 2017·ACS Biomaterials Science & Engineering·Natalie A ParlaneBernd H A Rehm

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