Effective equine immunization protocol for production of potent poly-specific antisera against Calloselasma rhodostoma, Cryptelytrops albolabris and Daboia siamensis

PLoS Neglected Tropical Diseases
Sompong SapsutthipasK Ratanabanangkoon

Abstract

Snake envenomation has been estimated to affect 1.8 million people annually with about 94,000 deaths mostly in poor tropical countries. Specific antivenoms are the only rational and effective therapy for these cases. Efforts are being made to produce effective, affordable and sufficient antivenoms for these victims. The immunization process, which has rarely been described in detail, is one step that needs to be rigorously studied and improved especially with regard to the production of polyspecific antisera. The polyspecific nature of therapeutic antivenom could obviate the need to identify the culprit snake species. The aim of this study was to produce potent polyspecific antisera against 3 medically important vipers of Thailand and its neighboring countries, namely Cryptelytrops albolabris "White lipped pit viper" (CA), Calleoselasma rhodostoma "Malayan pit viper" (CR), and Daboia siamensis "Russell's viper" (DS). Four horses were immunized with a mixture of the 3 viper venoms using the 'low dose, low volume multi-site' immunization protocol. The antisera showed rapid rise in ELISA titers against the 3 venoms and reached plateau at about the 8th week post-immunization. The in vivo neutralization potency (P) of the antisera a...Continue Reading

References

Jan 1, 1979·Toxicon : Official Journal of the International Society on Toxinology·R D Theakston, H A Reid
Jan 24, 1991·Journal of Immunological Methods·J Rungsiwongse, K Ratanabanangkoon
Jan 1, 1985·Toxicon : Official Journal of the International Society on Toxinology·J M GutiérrezL Cerdas
Jan 1, 1986·The American Journal of Tropical Medicine and Hygiene·C ViravanD A Warrell
Jan 1, 1983·Toxicon : Official Journal of the International Society on Toxinology·R D Theakston
Dec 1, 1994·Toxicon : Official Journal of the International Society on Toxinology·P G SellsD A Warrell
Mar 1, 1994·Toxicon : Official Journal of the International Society on Toxinology·G RojasJ M Gutiérrez
Jan 1, 1994·Journal of Toxicology. Clinical Toxicology·J M Scherrmann
May 1, 1994·Toxicon : Official Journal of the International Society on Toxinology·P Sunthornandh, K Ratanabanangkoon
Jul 8, 1998·Proceedings of the National Academy of Sciences of the United States of America·J BalsindeE A Dennis
Aug 2, 2001·Toxicon : Official Journal of the International Society on Toxinology·C ChotwiwatthanakunK Ratanabanangkoon
Dec 7, 2002·Toxicon : Official Journal of the International Society on Toxinology·Supod SriprapatKavi Ratanabanangkoon
Mar 27, 2003·Toxicon : Official Journal of the International Society on Toxinology·R A HarrisonR D G Theakston
Jul 9, 2003·Clinical Pharmacokinetics·José María GutiérrezBruno Lomonte
Feb 3, 2005·Toxicon : Official Journal of the International Society on Toxinology·Rutai Raweerith, Kavi Ratanabanangkoon
Aug 9, 2006·Toxicon : Official Journal of the International Society on Toxinology·A RialH Massaldi
Jan 19, 2008·Proteomics·Jay W Fox, Solange M T Serrano
Jul 16, 2008·Seminars in Nephrology·Talerngsak Kanjanabuch, Visith Sitprija
Oct 18, 2008·Toxicon : Official Journal of the International Society on Toxinology·Blanca Ramos-CerrilloRoberto P Stock
May 26, 2009·Toxicon : Official Journal of the International Society on Toxinology·E M El-KadyA F Wahby
Oct 31, 2009·Toxicon : Official Journal of the International Society on Toxinology·S B AbubakarUNKNOWN Nigeria-UK EchiTab Study Group
Dec 3, 2009·Toxicon : Official Journal of the International Society on Toxinology·José María GutiérrezDavid A Warrell
Dec 23, 2009·PLoS Neglected Tropical Diseases·Robert A HarrisonDavid G Lalloo
Jan 13, 2011·Toxicon : Official Journal of the International Society on Toxinology·Jean-Philippe Chippaux
May 3, 2011·Toxicon : Official Journal of the International Society on Toxinology·Irving G ArchundiaRoberto P Stock
Jul 5, 2011·Journal of Proteomics·Robert A HarrisonSimon C Wagstaff
Sep 15, 2012·Toxicon : Official Journal of the International Society on Toxinology·Choo Hock TanChristeine Ariaranee Gnanathasan

❮ Previous
Next ❯

Citations

Apr 9, 2016·PLoS Neglected Tropical Diseases·Kavi RatanabanangkoonNget Hong Tan
Jul 13, 2018·BioMed Research International·Urai Chaisri, Wanpen Chaicumpa
Jun 30, 2019·Toxicon : Official Journal of the International Society on Toxinology·Thava Malar Changra LingamChoo Hock Tan
Jun 3, 2021·Animals : an Open Access Journal From MDPI·Xavier Manteca VilanovaPatricia V Turner

❮ Previous
Next ❯

Methods Mentioned

BETA
Enzyme Linked Immuno-Sorbent Assay
ELISA
ELISAs

Related Concepts

Related Feeds

Avian Influenza: Innate Immune Adjuvant (ASM)

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.

Avian Influenza: Innate Immune Adjuvant

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.