Effective gene transfer to human melanomas via integrin-targeted adenoviral vectors

Human Gene Therapy
Takafumi NakamuraHirofumi Hamada

Abstract

The utility of recombinant adenoviral vectors (Adv) for gene therapy is limited by their low transduction efficiency and lack of specificity for target cells. The low transduction efficiency is often recognized as due to deficiency of the primary adenoviral receptor, the coxsackievirus-adenovirus receptor (CAR). In this paper, studies of CAR levels on human melanoma cell lines confirmed that low transduction efficiency was closely related to deficiency of the adenoviral receptor. To achieve CAR-independent gene transfer via Adv, we modified viral tropism via genetic alteration of the adenovirus type 5 (Ad5) fiber protein. Insertion of an Arg-Gly-Asp (RGD)-containing peptide in the HI loop of the fiber knob domain allowed the virus to use an alternative receptor, the integrin receptor, during the cell entry process. With this modified vector (Adv-F/RGD) transduction was increased 5- to 96-fold relative to a vector containing wild-type fiber (Adv-F/wt) in five human melanoma cells expressing integrins of the alpha(v)beta(3), alpha(v)beta(5) class, which are recognized by the RGD peptide motif. In contrast, no significant difference in transduction efficiency between Adv-F/RGD and Adv-F/wt was observed in 293 cells, which show hig...Continue Reading

References

Dec 20, 1989·Journal of the National Cancer Institute·W H ClarkA C Halpern
Oct 1, 1967·Journal of Virology·W C Lawrence, H S Ginsberg
May 31, 1994·Annals of the New York Academy of Sciences·S L Brody, R G Crystal
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·S H ChenS L Woo
Feb 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·S MiyakeI Saito
Jan 1, 1996·Annual Review of Cell and Developmental Biology·E Ruoslahti
Jan 13, 1997·Biochemical and Biophysical Research Communications·Y Yoshida, H Hamada
Apr 1, 1997·Proceedings of the National Academy of Sciences of the United States of America·R P TomkoL Philipson
Jun 1, 1997·Nature Biotechnology·R PasqualiniE Ruoslahti
Jul 1, 1997·Nature Medicine·C Nyberg-HoffmanE Aguilar-Cordova
Oct 6, 1997·Proceedings of the National Academy of Sciences of the United States of America·B M PützerF L Graham
Jun 9, 1998·Drugs·G L Cohen, C I Falkson
Mar 18, 2000·Cancer Metastasis Reviews·R E SeftorM J Hendrix
Jul 30, 1994·Breast Cancer : the Journal of the Japanese Breast Cancer Society·T WadaM Yasutomi

❮ Previous
Next ❯

Citations

Aug 12, 2006·Brain : a Journal of Neurology·H LiuJ D Kocsis
Jul 3, 2010·International Journal of Medical Sciences·Xiao-yan LiuMei-hua Sui
Dec 18, 2008·Cancer·Anita TandleSteven K Libutti
Oct 26, 2007·The Journal of Investigative Dermatology·Dennis HoffmannOliver Wildner
Nov 4, 2015·Frontiers in Neuroscience·Nafiseh Nafissi, Marianna Foldvari
Jun 1, 2005·The Journal of Gene Medicine·Hajime TsudaHirofumi Hamada
Jun 16, 2004·International Journal of Cancer. Journal International Du Cancer·Gerd J BauerschmitzAkseli Hemminki
Jun 10, 2005·The Journal of Gene Medicine·Hideyo UgaiKazunari K Yokoyama
Jan 6, 2007·International Journal of Cancer. Journal International Du Cancer·Kei TomiharaHirofumi Hamada
Aug 30, 2002·Biochemical and Biophysical Research Communications·L F ZerbiniA M Ventura
Oct 11, 2005·Biochemical and Biophysical Research Communications·Hideyo UgaiKazunari K Yokoyama
Dec 9, 2004·Molecular Therapy : the Journal of the American Society of Gene Therapy·Kazuhiko KurozumiHirofumi Hamada
Jul 6, 2004·Molecular Therapy : the Journal of the American Society of Gene Therapy·Hiroaki UchidaHirofumi Hamada
Feb 5, 2004·Molecular Therapy : the Journal of the American Society of Gene Therapy·Kazuhiko KurozumiHirofumi Hamada
Apr 2, 2003·Molecular Therapy : the Journal of the American Society of Gene Therapy·Hajime TsudaHirofumi Hamada
Oct 8, 2003·Molecular Therapy : the Journal of the American Society of Gene Therapy·Kazuhiro TakahashiHirofumi Hamada
Apr 21, 2017·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Darina RoitshtainNatan T Shaked
Apr 20, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Willem W OverwijkJohn B A G Haanen
Aug 11, 2020·Journal of Drug Targeting·Sepideh NezhadiFarid Dorkoosh
May 17, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Mariko WakayamaKazunari K Yokoyama
Jun 17, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Toshihiro TanakaHirofumi Hamada

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.