PMID: 7521509Jan 1, 1994Paper

Effective induction of human NK cells with OK-432 and further augmentation of their cytolytic function by rIL-2

Microbiology and Immunology
Z L HuT Katsuki

Abstract

Low concentrations of exogenously added recombinant interleukin 2 (rIL-2) were able to augment OK-432-induced natural killer (NK) cell activity. This kind of augmenting effect depended on the dose of rIL-2 and manifested itself only in PBMC stimulated with OK-432 (OK-MC) followed by rIL-2; augmentation did not happen in the reverse order. The existence of CD16+/CD25+ (IL-2 receptor positive; IL-2R+) and CD57+/CD25+ double positive cells which possess NK cell surface markers in OK-MC markedly increased in a long-term culture (12 days). A strong positive correlation was observed between the IL-2-dependent augmentation of NK activity and the quantitative changes in cell populations that possessed NK cell phenotypes. Treatment of the day-12-OK-MC with monoclonal anti-CD56 antibody plus complement could almost completely abrogate the augmented NK cytotoxicity. Furthermore, this augmenting effect was detectable within 4 hr after addition of rIL-2 at single cell level, suggesting that the effect did not require NK cell's DNA synthesis. Thus it was suggested that OK-432 could promote and upregulate the expression of IL-2 receptor (CD25) on CD56+ NK cell populations. Moreover, it was considered that the interaction of low concentration ...Continue Reading

References

Jan 1, 1981·International Journal of Immunopharmacology·A Uchida, M Micksche
Jan 1, 1984·International Archives of Allergy and Applied Immunology·M De Ley, H Claeys
Oct 1, 1984·The Journal of Experimental Medicine·G TrinchieriB Perussia
Jan 1, 1993·Journal of Cranio-maxillo-facial Surgery : Official Publication of the European Association for Cranio-Maxillo-Facial Surgery·E KubotaJ Tadano

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Citations

Jul 9, 2002·International Journal of Dermatology·Hiroyuki MiuraKunihiko Yoshikawa
May 1, 1997·The Journal of Dermatology·S InuiK Yoshikawa

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