Effective inhibition of nitric oxide production by aminoguanidine does not reverse hypotension in endotoxaemic rats

Acta Anaesthesiologica Scandinavica
K MetcalfB Lisander

Abstract

Excess production of nitric oxide (NO) by the inducible NO synthase (iNOS) has been implicated in the pathophysiology of septic shock. Using methaemoglobin (metHb) and the stable NO metabolite nitrate as markers of NO formation, we assessed the effect of iNOS blockade by aminoguanidine (AG) on hypotension and NO formation in endotoxaemic rats. In 32 male Wistar rats under chloralose anaesthesia, MetHb (at 15 and 330 min, respectively) and plasma nitrate (at 330 min) were determined. Mean arterial pressure, heart rate and haematocrit were monitored. The LPS group (n=8) received bacterial endotoxin (LPS), 3 mg kg(-1) i.v. and was subsequently monitored for 5 h. At 2 h after LPS, the LPS+AG20 group (n=8) received AG, 5 mg kg(-1), and 5 mg kg(-1) h(-1) for the remaining 3 h. The LPS+AG100 group (n=8) instead received 25 mg kg(-1), followed by 25 mg kg(-1) h(-1). The NaCl group (n=8) was given corresponding volumes of isotonic saline. AG decreased the LPS-induced rise in plasma nitrate by about 50% in the LPS+AG20 group. MetHb levels, however, were not appreciably reduced by this dose. Both NO metabolites reached control levels after the higher dose of AG. LPS caused a progressive decrease in haematocrit. AG did not influence the LP...Continue Reading

References

Nov 1, 1993·British Journal of Pharmacology·M J GriffithsT W Evans
Dec 1, 1994·American Journal of Respiratory and Critical Care Medicine·C M Martin, W J Sibbald
Jan 10, 1995·Archives of Biochemistry and Biophysics·D J Wolff, A Lubeskie
Apr 1, 1995·British Journal of Pharmacology·U N WestfeltA Wennmalm
May 1, 1997·The Biochemical Journal·G BenthinA Wennmalm
Feb 4, 1998·Hypertension·D L MattsonA W Cowley
Jul 11, 1998·Critical Care Medicine·C M Pastor, P M Suter
Oct 17, 1998·Acta Anaesthesiologica Scandinavica·K MetcalfB Lisander
Mar 19, 1999·Acta Anaesthesiologica Scandinavica·K A Kirkebøen, O A Strand
May 8, 1999·Journal of Applied Physiology·J A Scott, D G McCormack
Jun 14, 2000·American Journal of Respiratory and Critical Care Medicine·J L VincentJ C Preiser
Dec 9, 2000·American Journal of Respiratory and Critical Care Medicine·S LanoneJ Boczkowski
Jan 11, 2001·Acta Anaesthesiologica Scandinavica·K Metcalf, B Lisander

❮ Previous
Next ❯

Citations

Jun 26, 2003·Clinical and Experimental Pharmacology & Physiology·Alessia PedotoEnrico M Camporesi
May 28, 2005·The Journal of Trauma·Kerstin MetcalfBjorn Lisander
Apr 24, 2008·Critical Care Medicine·Heidrun FinkManfred Blobner
Jan 27, 2005·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·C-Y LiuM E Kreis
Oct 11, 2012·Pharmacology, Biochemistry, and Behavior·Deidiane E RibeiroAlexandre Giusti-Paiva
Aug 9, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Fumito IchinoseWarren M Zapol
Feb 19, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Thomas J CalvertLeif D Nelin
Jul 15, 2018·Pflügers Archiv : European journal of physiology·Stefan J SchallerJ A Jeevendra Martyn

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.