Effective Interactions and Colloidal Stability of Bovine γ-Globulin in Solution

The Journal of Physical Chemistry. B
Stefano Da VelaF Schreiber

Abstract

Interactions and phase behavior of γ-globulins are of fundamental interest in biophysical and pharmaceutical research, as these are among the most abundant proteins in blood plasma. In this work, we report the characterization of the oligomeric state of bovine γ-globulin, the effective protein-protein interactions, and the colloidal stability in aqueous solution as a function of protein concentration and ionic strength. Classical biochemical techniques, such as size exclusion chromatography (SEC) and gel electrophoresis, together with small-angle X-ray and neutron scattering (SAXS/SANS), were employed for this study. The results show that bovine γ-globulin solutions are dominated by monomer and idiotype anti-idiotype dimer. Despite the flexibility and highly nonspherical shape of the protein, a simple model with a disk-type form factor and a structure factor of a square-well potential provide a satisfying description of the scattering data. The overall interactions are attractive and the strength decreases with increasing protein concentration, or adding buffer or salts. For higher protein volume fraction (>7%), the model would imply a strong particle-particle correlation which does not appear in the experimental data. This mis...Continue Reading

References

Apr 1, 1988·Journal of Protein Chemistry·T JøssangE Rosenqvist
Jan 1, 1988·Molecular Immunology·D L TankersleyJ S Finlayson
Mar 29, 1994·Journal of Immunological Methods·M Plebanski, S S Burtles
Mar 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·N BanA McPherson
Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·D I SvergunG Zaccai
May 24, 2001·Proceedings of the National Academy of Sciences of the United States of America·A PandeG B Benedek
Sep 28, 2002·Acta Crystallographica. Section D, Biological Crystallography·Annette TardieuDenis Vivarès
Oct 23, 2002·Proceedings of the National Academy of Sciences of the United States of America·Onofrio AnnunziataGeorge B Benedek
Mar 4, 2003·Nature Medicine·Thomas A Waldmann
Jan 27, 2004·Journal of Molecular Biology·Oleg Galkin, Peter G Vekilov
Feb 19, 2004·Methods in Molecular Biology·Paul Cutler
Mar 11, 2004·The European Physical Journal. E, Soft Matter·D VivarèsF Bonneté
Jul 21, 2004·Journal of Computational Chemistry·Eric F PettersenThomas E Ferrin
Sep 24, 2005·Clinical and Experimental Immunology·S JollesS A Misbah
Jun 8, 2006·Annual Review of Biochemistry·Fabrizio Chiti, Christopher M Dobson
Jul 20, 2006·The Journal of Chemical Physics·Andrey ShiryayevJ D Gunton
Sep 29, 2006·The Journal of Physical Chemistry. B·Sylvain GrouazelStéphane Veesler
Feb 6, 2007·Molecular Immunology·Peter GronskiErnst-Jürgen Kanzy
Apr 24, 2007·Biophysical Journal·Tangir AhamedJörg Thömmes
Dec 17, 2008·Journal of Pharmaceutical Sciences·Joshua D RamseyC Russell Middaugh
Apr 24, 2010·Nature Reviews. Immunology·Louis M WeinerShangzi Wang
Feb 12, 2011·Biotechnology Progress·Rachael A LewusStanley I Sandler
Feb 23, 2011·Scandinavian Journal of Immunology·S WymannS M Miescher
Apr 6, 2012·Pharmaceutical Research·Charlotte Rode MosbækBente Vestergaard
Jul 26, 2012·The Journal of Physical Chemistry. B·Wayne G LilyestromThomas M Scherer
Aug 1, 2012·Proceedings of the National Academy of Sciences of the United States of America·Ying WangGeorge B Benedek
Feb 16, 2013·Nature Reviews. Immunology·Inessa Schwab, Falk Nimmerjahn
Mar 6, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Yunfeng YanPaul L Dubin
Apr 9, 2013·The Journal of Physical Chemistry. B·Wayne G LilyestromThomas M Scherer
Apr 9, 2013·Biophysical Journal·Jessica BalboRebecca C Wade
Aug 6, 2013·Molecular Immunology·Ertan EryilmazArturo Casadevall
Oct 4, 2013·Journal of Molecular Recognition : JMR·Rui C ChavesJean-Luc Pellequer

❮ Previous
Next ❯

Citations

Apr 20, 2018·Emerging Topics in Life Sciences·Martin A Schroer, Dmitri I Svergun
Oct 19, 2017·Biophysical Journal·Jacob B RoweYing Wang
Mar 22, 2019·The Journal of Physical Chemistry Letters·Marco GrimaldoFrank Schreiber
Nov 22, 2018·The Journal of Physical Chemistry. B·Michal K BraunFrank Schreiber
Oct 13, 2021·Molecular Pharmaceutics·Anita GirelliFelix Roosen-Runge

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.