Effective mobility as a robust criterion for compound annotation and identification in metabolomics: Toward a mobility-based library

Analytica Chimica Acta
Nicolas DrouinSerge Rudaz

Abstract

Capillary electrophoresis (CE) presents many advantageous features as an analytical technique in metabolomics, such as very low consumption of a sample or the possibility to easily detect very polar and ionizable compounds. However, CE remains an approach only used by a few research groups due to a relatively lower sensitivity and, higher analysis time compared to liquid chromatography. To circumvent these drawbacks, herein we propose a generic CE-mass spectrometry (MS) approach using positive electrospray ionization mode and performing normal- and reverse-polarity CE separations to analyze anionic and acidic compounds. Preliminary experiments showed better sensitivity using the ESI positive mode compared to the ESI negative mode on a set of representative anionic compounds from different biochemical families. This approach was applied to the investigation of an available library of metabolites. More than 450 compounds out of the 596 in the library were detected, with the possibility to monitor negatively ionizable compounds through their ammonium adducts. Migration time of each data point was converted to an effective mobility (μeff) scale and used for peak alignment in data pre-processing; μeff features were used as a robust ...Continue Reading

Citations

May 21, 2019·Electrophoresis·Marlien van MeverRawi Ramautar
Sep 10, 2020·Electrophoresis·Wei Zhang, Rawi Ramautar
Apr 30, 2019·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Javier Sastre TorañoGerhardus de Jong
Jul 4, 2021·Electrophoresis·Santiago CodesidoVíctor González-Ruiz
Nov 9, 2019·Analytical Chemistry·Courtney J KristoffLisa A Holland

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