Effective oncolytic vaccinia therapy for human sarcomas.

The Journal of Surgical Research
Shuangba HeRichard J Wong

Abstract

Approximately one fourth of bone and soft-tissue sarcomas recur after prior treatment. GLV-1h68 is a recombinant, replication-competent vaccinia virus that has been shown to have oncolytic effects against many human cancer types. We sought to determine whether GLV-1h68 could selectively target and lyse a panel of human bone and soft-tissue sarcoma cell lines in vitro and in vivo. GLV-1h68 was tested in a panel of four cell lines including: fibrosarcoma HT-1080, osteosarcoma U-2OS, fibrohistiocytoma M-805, and rhabdomyosarcoma HTB-82. Gene expression, infectivity, viral proliferation, and cytotoxicity were characterized in vitro. HT-1080 xenograft flank tumors grown in vivo were injected intratumorally with a single dose of GLV-1h68. All four cell lines supported robust viral transgene expression in vitro. At a multiplicity of infection (MOI) of five, GLV-1h68 was cytotoxic to three cell lines, resulting in >80% cytotoxicity over 7 d. In vivo, a single injection of GLV-1h68 into HT-1080 xenografts exhibited localized intratumoral luciferase activity peaking at d 2-4, with gradual resolution over 8 d and no evidence of spread to normal tissues. Treated animals exhibited near-complete tumor regression over a 28-d period without ob...Continue Reading

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Citations

Apr 4, 2015·Annual Review of Virology·Winnie M Chan, Grant McFadden
Sep 21, 2013·Cell Stress & Chaperones·Jürgen Radons
Jan 16, 2016·Viruses·Evgeniy ZonovElena Ryabchikova
Jan 18, 2014·Gynecologic Oncology·Yu-Ping LiuKah-Whye Peng
Feb 3, 2016·Oncology Letters·Jia WanXiangsheng Zhang

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