PMID: 10414030Jul 22, 1999

Effective rate models for the analysis of transport-dependent biosensor data

Mathematical Biosciences
T MasonB Goldstein

Abstract

Optical biosensors, including the BIACORE, provide an increasingly popular method for determining reaction rates of biomolecules. In a flow chamber, with one reactant immobilized on a chip on the sensor surface, a solution containing the other reactant (the analyte) flows through the chamber. The time course of binding of the reactants is monitored. Scientists using the BIACORE to understand biomolecular reactions need to be able to separate intrinsic reaction rates from the effects of transport in the biosensor. For a model to provide a useful basis for such an analysis, it must reflect transport accurately, while remaining simple enough to couple with a routine for estimating reaction rates from BIACORE data. Models have been proposed previously for this purpose, consisting of an ordinary differential equation with 'effective rate coefficients' incorporating reaction and transport parameters. In this paper we investigate both the theoretical basis and numerical accuracy of these and related models.

References

Nov 1, 1977·Biophysical Journal·H C Berg, E M Purcell
Sep 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·C DeLisi, F W Wiegel
Dec 1, 1996·Biophysical Journal·J P BrodyR H Austin
Dec 1, 1996·Protein Science : a Publication of the Protein Society·D G MyszkaT L Ciardelli

Citations

Nov 11, 1999·Journal of Molecular Recognition : JMR·B GoldsteinCarla Wofsy
Dec 13, 2000·Journal of Molecular Recognition : JMR·R L Rich, D G Myszka
Mar 30, 2001·Bulletin of Mathematical Biology·David A Edwards
Jun 24, 2006·Bulletin of Mathematical Biology·David A Edwards
Feb 19, 2000·Current Opinion in Biotechnology·R L Rich, D G Myszka
Sep 30, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Craig S SchneiderAnthony J Kim
Jul 20, 2014·Bulletin of Mathematical Biology·Matthew E Zumbrum, David A Edwards
Apr 5, 2014·Journal of Molecular Recognition : JMR·Massinissa Si MehandBala Srinivasan
Nov 22, 2005·Analytical Biochemistry·D Keith Roper, Shamit Nakra
Sep 11, 2014·Journal of Mathematical Biology·Matthew E Zumbrum, David A Edwards
Sep 22, 2005·Lab on a Chip·Andrea LionelloHubert H Girault
Apr 11, 2006·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Marie A Iannone, Thomas G Consler
Mar 7, 2006·The Journal of Biological Chemistry·Shallee PageAndré Bensadoun
Mar 29, 2020·Micromachines·Marwa Selmi, Hafedh Belmabrouk
Dec 13, 2000·Journal of Molecular Recognition : JMR·R L Rich, D G Myszka
Mar 11, 2018·Biochemistry. Biokhimii︠a︡·D V SotnikovB B Dzantiev

Related Concepts

Ligands
Numerical Analysis, Computer-Assisted
Biosensing Techniques

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

The Tendon Seed Network

Tendons are rich in the extracellular matrix and are abundant throughout the body providing essential roles including structure and mobility. The transcriptome of tendons is being compiled to understand the micro-anatomical functioning of tendons. Discover the latest research pertaining to the Tendon Seed Network here.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Incretins

Incretins are metabolic hormones that stimulate a decrease in glucose levels in the blood and they have been implicated in glycemic regulation in the remission phase of type 1 diabetes. Here is the latest research.

Chromatin Regulation and Circadian Clocks

The circadian clock plays an important role in regulating transcriptional dynamics through changes in chromatin folding and remodelling. Discover the latest research on Chromatin Regulation and Circadian Clocks here.

Long COVID-19

“Long Covid-19” describes illness in patients who are reporting long-lasting effects of the SARS-CoV-19 infection, often long after they have recovered from acute Covid-19. Ongoing health issues often reported include low exercise tolerance and breathing difficulties, chronic tiredness, and mental health problems such as post-traumatic stress disorder and depression. This feed follows the latest research into Long Covid.

Spatio-Temporal Regulation of DNA Repair

DNA repair is a complex process regulated by several different classes of enzymes, including ligases, endonucleases, and polymerases. This feed focuses on the spatial and temporal regulation that accompanies DNA damage signaling and repair enzymes and processes.