Effective Selection of a Well-Differentiated Type of Human Uterine Endometrial Carcinoma Cells by Transfection of the Sulfotransferase Gene and Possible Association of Sulfoglycolipids With Well-Differentiated Phenotypes

International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society
Kyoko TanakaM Iwamori

Abstract

Sulfatide has been shown to be characteristically increased on the apical surface of the normal endometrium at the secretory phase, and to be related with the formation of the glandular structure and the secretion of mucin from glands for the implantation of a fertilized egg. Additionally, sulfatides are expressed in the well-differentiated type, but not in the poorly differentiated type, of endometrial carcinomas. This suggests that sulfatides are a molecular marker of differentiated phenotypes. To further elucidate the biological significance of sulfoglycolipids, we transfected the sulfotransferase gene into endometrial carcinoma-derived cells without sulfoglycolipids and compared their glycolipid compositions and phenotypes with those of the original cells. The glycolipid sulfotransferase gene was transfected into endometrial carcinoma-derived SNG-II cells, the resultant transfected cells being found to frequently form a domelike structure, and some of them were selected as SNG-II-GST cells. We compared the glycolipid compositions and phenotypes of SNG-II and SNG-II-GST cells. Although the original SNG-II cells grew in a paving stone pattern, SNG-II-GST cells formed a domelike structure. SNG-II-GST cells exhibited high GST a...Continue Reading

References

Jul 15, 1991·Biochemical and Biophysical Research Communications·M MomoedaY Nagai
Dec 15, 1986·Chemistry and Physics of Lipids·D D Roberts
Aug 18, 1997·Biochemical and Biophysical Research Communications·M IwamoriN Ito
Jun 26, 1998·Progress in Lipid Research·I Ishizuka
May 13, 2008·Glycoconjugate Journal·Ken-Ichi NagaiIneo Ishizuka
Nov 20, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sae-Bom JeonEun Jung Park
Jul 18, 2012·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Taro SugiyamaMasao Iwamori

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