Effective separation of peptides using highly dense thermo-responsive polymer brush-grafted porous polystyrene beads

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Aya MizutaniTeruo Okano

Abstract

For the development of well-defined highly dense thermo-responsive polymer grafted surface as an improved stationary phase for thermo-responsive chromatography, poly(N-isopropylacrylamide) (PIPAAm) brush-grafted porous polystyrene beads were prepared by surface-initiated atom transfer radical polymerization (ATRP). The PIPAAm grafted region of polystyrene beads was adjusted by the addition of isooctane as a poor solvent for polystyrene upon the reaction of ATRP initiator immobilization. Using a thermo-responsive HPLC column containing the prepared beads with PIPAAm brush grafted on the inside pores nearby the outer surfaces, angiotensin subtypes were effectively separated with aqueous mobile phase, because the densely grafted PIPAAm on nearby the outer surface effectively interacted with the peptides hydrophobically. Retention of basic peptide was achieved by the beads with basic mobile phase. These results indicated that the prepared beads with grafted PIPAAm nearby the outer surface became an effective chromatographic stationary phase for retaining basic peptides using wide pH range of mobile phase.

References

Apr 20, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Carmen Alvarez-Lorenzo, Angel Concheiro
Dec 28, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Naokazu IdotaTeruo Okano
Aug 9, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Kenichi NagaseTeruo Okano
Dec 19, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Kenichi NagaseTeruo Okano

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Citations

Jun 15, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Changying XueDeborah E Leckband
Apr 26, 2015·Analytical and Bioanalytical Chemistry·Rosa A LorenzoCarmen Alvarez-Lorenzo
Jul 8, 2015·Journal of Chromatography. a·Alexandros LamprouMassimo Morbidelli
May 6, 2016·Biomaterials Science·Young-Jin KimYukiko T Matsunaga
Aug 3, 2016·Chemical Communications : Chem Comm·Ko MatsukawaRyo Yoshida
Jan 15, 2014·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Eri Ayano, Hideko Kanazawa
Oct 21, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Kenichi Nagase, Teruo Okano
Nov 10, 2020·Analytica Chimica Acta·Kenichi Nagase, Hideko Kanazawa
Jan 16, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Sinuo TanMilton T W Hearn

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