Effective stimulation of growth in MCF-7 human breast cancer cells by inhibition of syntaxin18 by external guide sequence and ribonuclease P

Cancer Letters
Tyler BassettChow H Lee

Abstract

Syntaxin18 (Stx18) is an endoplasmic reticulum (ER)-membrane bound SNARE protein involved in membrane trafficking between the ER and Golgi as well as in phagocytosis. Stx18 has also been shown to physically interact with proteins involved in the cell cycle and apoptosis. These findings suggest the possible role of Stx18 in regulating cell growth. In this study, we used theoretically designed external guide sequence molecule which utilizes RNase P to cleave Stx18 mRNA and down-regulate Stx18 levels in MCF-7 human breast cancer cells. We showed that down-regulation of Stx18 leads to significant enhancement of growth in MCF-7 cells. Consistent with this finding was the observation that over-expression of Stx18 using the CMV promoter led to suppression of cell growth. Over-expressing Stx18 had no effect on c-myc mRNA expression and half-life, suggesting that the mechanism does not involve control at the transcriptional and post-transcriptional level of the c-myc gene. Finally, we showed that Stx18 is over-expressed in clinical human breast cancer. Overall, this study showed that Stx18 plays a role in the growth of human breast cancer cells and provided the basis for further investigation in determining whether it can be used as a p...Continue Reading

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Citations

Jan 21, 2010·European Journal of Human Genetics : EJHG·Roxann G IngersollTerri H Beaty
Nov 23, 2013·Journal of Mammary Gland Biology and Neoplasia·Sandrine TruchetMichèle Ollivier-Bousquet
Mar 10, 2010·New Biotechnology·Eirik Wasmuth Lundblad, Sidney Altman
May 16, 2009·The International Journal of Biochemistry & Cell Biology·Ge Shan
Apr 14, 2015·Annals of the New York Academy of Sciences·Carol Davies-SalaMarcelo E Tolmasky
May 26, 2016·Asian Pacific Journal of Cancer Prevention : APJCP·E Lara-PadillaC Bandala

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