Effectiveness factor and diffusion limitations in collagen gel modules containing HepG2 cells.

Journal of Tissue Engineering and Regenerative Medicine
Lindsay Corstorphine, Michael V Sefton

Abstract

A major obstacle in tissue engineering is overcoming hypoxia in thick, three-dimensional (3D) engineered tissues, which is caused by the diffusional limitations of oxygen and lack of internal vasculature to facilitate mass transfer. Modular tissue engineering is a bio-mimetic strategy that forms scalable, vascularized and uniform 3D constructs by assembling small (sub-mm), cell-containing modules. It was previously assumed that mass transfer resistance within the individual modules was negligible, due to their small size. In the present study, this assumption was tested using theoretical analysis of oxygen transport within the module (effectiveness factor) and experimental studies. Small (400 µm diameter post-contraction) and large (700 µm diameter post-contraction) HepG2-collagen modules were made for a range of seeding densities (2 × 10(6) -1 × 10(7) cells/ml collagen). Cell density, distribution and morphology within the modules showed that the small modules were capable of sustaining high cell densities (8.0 × 10(7) ± 4.4 × 10(7) cells/cm(3) ) with negligible mass transfer inhibition. Conversely, large modules developed a necrotic core and had significantly (p < 0.05) reduced cell densities (1.5 × 10(7) ± 9.2 × 10(6) cells/...Continue Reading

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Citations

Dec 24, 2010·Journal of Visualized Experiments : JoVE·M Dean ChamberlainMichael V Sefton
Feb 10, 2016·Proceedings of the National Academy of Sciences of the United States of America·Xuanyi MaShaochen Chen
Jul 26, 2011·Biomaterials·Christopher S SzotMarissa N Rylander
Feb 11, 2015·Tissue Engineering. Part a·David M KepecsRichard E Gilbert
Oct 26, 2016·Journal of Bioscience and Bioengineering·Takayuki TakeiMasahiro Yoshida
Jan 18, 2012·Cells, Tissues, Organs·Allison P Van WinkleMichael S Kallos
Nov 16, 2018·Tissue Engineering. Part C, Methods·Katherine A CumminsDavid K Wood
Jun 1, 2017·Current Stem Cell Reports·Peter R CorridonAnthony Atala
Apr 24, 2020·Frontiers in Bioengineering and Biotechnology·Annalisa TirellaNicola Tirelli
Jan 2, 2020·APL Bioengineering·Gregory H Underhill, Salman R Khetani
Nov 12, 2018·ACS Biomaterials Science & Engineering·Redouan MahouMichael V Sefton

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