Effectiveness of a tris-based extender (SHOTOR diluent) for the preservation of Bactrian camel (Camelus bactrianus) semen

Cryobiology
A Niasari-NaslajiA Gerami

Abstract

The development of a suitable semen extender is required to extend artificial breeding programs and to preserve the genetic potential of Bactrian camel. Experiments were conducted to provide the optimal osmolality and pH of tris-based extender and to compare that with available extenders for short-term preservation of Bactrian camel semen at 4 degrees C during 24 h. In experiments I and II, the effects of varying osmolalities (270, 300, 330, 360, and 390 mOsm/kg) and pHs (5.5, 6, 6.9, 7.5, 7.9, and 8.9) of tris-based extender on sperm viability were investigated. In experiment III, the efficiency of tris-based extender (SHOTOR diluent) in preserving Bactrian camel semen was compared with lactose (10%), sucrose (10%) and Green buffer. Viability parameters including progressive forward motility (PFM), plasma membrane integrity and the percentage of live spermatozoa were assessed. The data were analyzed using general linear model procedure. In the majority of assessments using tris-based extender, the viability of spermatozoa was superior at the osmolality of 330 mOsm/kg and pH of 6.9. PFM was significantly greater at the time of semen dilution in tris-based (65.5%) and Green buffer (60.5%) compared to that of lactose (31%) and su...Continue Reading

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Citations

Nov 17, 2012·Animal Reproduction Science·J A SkidmoreM Billah
Aug 1, 2012·Reproduction in Domestic Animals = Zuchthygiene·C M Kershaw-Young, W M C Maxwell
Dec 3, 2014·Animal Reproduction Science·S ZiapourH Adel
Nov 23, 2019·Animals : an Open Access Journal From MDPI·Ayman Abdel-Aziz SwelumHany Abdalla
Oct 1, 2019·Zygote : the Biology of Gametes and Early Embryos·Vahid VahediNemat Hedayat Evrigh
Jan 8, 2021·Tropical Animal Health and Production·Mohamed A ShahinMostafa A El-Harairy

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