Effectiveness of charged noncovalent polymer coatings against protein adsorption to silica surfaces studied by evanescent-wave cavity ring-down spectroscopy and capillary electrophoresis

Analytical Chemistry
R HaselbergG W Somsen

Abstract

Protein adsorption to silica surfaces is a notorious problem in analytical separations. Evanescent-wave cavity ring-down spectroscopy (EW-CRDS) and capillary electrophoresis (CE) were employed to investigate the capability of positively charged polymer coatings to minimize the adsorption of basic proteins. Adsorption of cytochrome c (cyt c) to silica coated with a single layer of polybrene (PB), or a triple layer of PB, dextran sulfate (DS), and PB, was studied and compared to bare silica. Direct analysis of silica surfaces by EW-CRDS revealed that both coatings effectively reduce irreversible protein adsorption. Significant adsorption was observed only for protein concentrations above 400 microM, whereas the PB-DS-PB coating was shown to be most effective and stable. CE analyses of cyt c were performed with and without the respective coatings applied to the fused-silica capillary wall. Monitoring of the electroosmotic flow and protein peak areas indicated a strong reduction of irreversible protein adsorption by the positively charged coatings. Determination of the electrophoretic mobility and peak width of cyt c revealed reversible protein adsorption to the PB coating. It is concluded that the combination of results from EW-CR...Continue Reading

References

Feb 10, 1998·Electrophoresis·B GasE Kenndler
Sep 29, 2006·The Journal of Physical Chemistry. B·Michael A EverestHolly A Neill
Dec 7, 2007·Electrophoresis·Vladislav Dolník
Jan 1, 2008·Journal of Chromatography. a·Charles A LucyMakedonka D Gulcev
Jun 27, 2009·Journal of Separation Science·Rob HaselbergGovert W Somsen
Jul 20, 2010·Annual Review of Analytical Chemistry·L van der SneppenW Ubachs

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Citations

Jul 24, 2012·Electrophoresis·Glareh Azadi, Anubhav Tripathi
Jul 30, 2010·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Hayley V PowellPatrick R Unwin
Oct 5, 2010·Chemical Society Reviews·Mathias SchnipperingPatrick R Unwin
Mar 22, 2014·Soft Matter·David A Woods, Colin D Bain
Apr 12, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Michael A O'ConnellPatrick R Unwin

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