Effectiveness of fructose-modified chitosan as a scaffold for hepatocyte attachment

Biological & Pharmaceutical Bulletin
K YagiM Kawase

Abstract

Free amino groups of chitosan, a substance which has previously been shown to be a good scaffold for hepatocyte attachment, were covalently modified with fructose. The modification significantly increased the number of cells that could be attached on the surface of chitosan gel. Rat hepatocytes cultivated on fructose-chitosan behaved similarly to those on unmodified chitosan, i.e., they retained the spherical shape they have in vivo, and released much less lactate dehydrogenase than cells attached on a collagen-coated surface. The modification with fructose did not alter the important characteristics of chitosan for hepatocyte culture: liver-specific functions such as urea synthesis and drug metabolism were stably maintained for 5 d in the hepatocytes cultured on fructose-chitosan. In sharp contrast, hepatocytes attached on a collagen-coated surface underwent a severe morphological change, from spherical to flat, and lost almost all their lidocaine-removal activity within 5d. A very thin fructose-chitosan layer was also applied onto the collagen-coated surfaces of polystyrene plates and a dextran microcarrier by crosslinking free amino groups in the chitosan and collagen with glutaraldehyde to fix the thin layer. Hepatocytes on...Continue Reading

Citations

Apr 30, 2013·Acta Biomaterialia·Sarah WassmerCatherine Tsilfidis
Aug 1, 2007·Journal of Materials Science. Materials in Medicine·Chen WuYaoting Yu
Sep 29, 2011·Hepatobiliary & Pancreatic Diseases International : HBPD INT·Xu-Bo WuBao-San Han
Jan 8, 2010·Cytotherapy·Ming-Hua ZhengYong-Ping Chen
Nov 8, 2003·The Journal of Pharmacy and Pharmacology·Corinne PlanchampBernard Testa
Jul 25, 2003·Biochemical and Biophysical Research Communications·Toshio TsutsuiKiyohito Yagi
May 12, 2005·Biomaterials·Narayan BhattaraiMiqin Zhang
Jul 15, 2005·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Xiaohong WangQingping Lu
Jan 15, 2005·Journal of Biomaterials Science. Polymer Edition·Seog-Jin SeoChong-Su Cho
Feb 22, 2002·Biotechnology and Bioengineering·Jun Jin YoonTae Gwan Park
Nov 13, 2003·Journal of Biomedical Materials Research. Part a·Jieliang LiYaoting Yu
Apr 25, 2000·Journal of Periodontology·Y M LeeC P Chung
Feb 19, 2005·Macromolecular Bioscience·Narayan BhattaraiMiqin Zhang
Sep 15, 2004·Tissue Engineering·Paul LinSangeeta N Bhatia
Nov 19, 2010·Tissue Engineering. Part B, Reviews·Yella MartinJustin R Sharpe
Apr 19, 2008·Journal of Biomaterials Science. Polymer Edition·Feng ChenYoshito Ikada
Jul 22, 2009·Journal of Biomaterials Science. Polymer Edition·Rui ChenXiumei Mo
Oct 31, 2009·Journal of Biomaterials Science. Polymer Edition·Feng ChenYoshito Ikada
Jun 23, 2010·Journal of Biomaterials Science. Polymer Edition·Junichiro SarukawaHiroshi Tamura
Jun 28, 2001·Journal of Biomedical Materials Research·M KawaseK Yagi
Sep 27, 2016·Advanced Science·Anwarul HasanArghya Paul
Jul 6, 2000·Journal of Biomedical Materials Research·A LahijiC G Frondoza
Jun 14, 2020·International Journal of Biological Macromolecules·Katarzyna Adamiak, Alina Sionkowska
Nov 17, 2001·Chemical Reviews·K Y Lee, D J Mooney

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.