Effectiveness of ZnPc and of an amine derivative to inactivate Glioblastoma cells by Photodynamic Therapy: an in vitro comparative study

Scientific Reports
Fabiola N VelazquezCésar G Prucca

Abstract

Glioblastoma multiforme is considered to be one of the most aggressive types of tumors of the central nervous system, with a poor prognosis and short survival periods of ~ one year. The current protocol for glioblastoma treatment includes the surgical excision of the primary tumor followed by radio and chemotherapy. Photodynamic therapy (PDT) is considered a promising strategy for the treatment of several types of tumors. Phthalocyanines (Pcs) are good photosensitizers (PSs) for PDT because they induce cell death in several cellular models. ZnPc (Zn(II)phthalocyanine) is a well-known Pc, extensively tested in different cells and tumor models, but its evaluation on a glioblastoma model has been poorly studied. Herein, we compare the capacity of ZnPc and one of its derivatives, Zn(II)tetraminephthalocyanine (TAZnPc), to photoinactivate glioblastoma cells (T98G, MO59, LN229 and U87-MG) in culture. We measured the cellular uptake, the toxicity in the dark and the subcellular localization of the different Pcs, as well as the clonogenic capacity of surviving cells after PDT. The mechanism of cell death induced after PDT was determined by measuring caspase 3 activation, DNA fragmentation, phosphatidylserine externalization, mitochondr...Continue Reading

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Citations

Dec 29, 2020·Journal of Neuroscience Research·Luis Exequiel IbarraLaura Natalia Milla Sanabria
Jul 4, 2020·Anti-cancer Agents in Medicinal Chemistry·Giftson J SenapathyHeidi Abrahamse

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Methods Mentioned

BETA
surgical resection
flow cytometry
confocal microscopy
Fluorescence
Assay
electrophoresis

Software Mentioned

ImageJ
Image J
Huygens Deconvolution

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