Effector discovery in the fungal wheat pathogen Zymoseptoria tritici

Molecular Plant Pathology
Amir Mirzadi GohariGert H J Kema

Abstract

Fungal plant pathogens, such as Zymoseptoria tritici (formerly known as Mycosphaerella graminicola), secrete repertoires of effectors to facilitate infection or trigger host defence mechanisms. The discovery and functional characterization of effectors provides valuable knowledge that can contribute to the design of new and effective disease management strategies. Here, we combined bioinformatics approaches with expression profiling during pathogenesis to identify candidate effectors of Z. tritici. In addition, a genetic approach was conducted to map quantitative trait loci (QTLs) carrying putative effectors, enabling the validation of both complementary strategies for effector discovery. In planta expression profiling revealed that candidate effectors were up-regulated in successive waves corresponding to consecutive stages of pathogenesis, contrary to candidates identified by QTL mapping that were, overall, expressed at low levels. Functional analyses of two top candidate effectors (SSP15 and SSP18) showed their dispensability for Z. tritici pathogenesis. These analyses reveal that generally adopted criteria, such as protein size, cysteine residues and expression during pathogenesis, may preclude an unbiased effector discover...Continue Reading

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Citations

May 6, 2016·Frontiers in Plant Science·Graeme J Kettles, Kostya Kanyuka
Jun 21, 2015·Fungal Genetics and Biology : FG & B·Alison TestaJames Hane
Jun 21, 2015·Fungal Genetics and Biology : FG & B·Andrea Sánchez-ValletBruce A McDonald
Jun 21, 2015·Fungal Genetics and Biology : FG & B·Gero Steinberg
Jun 21, 2015·Fungal Genetics and Biology : FG & B·Sarrah Ben M'BarekGerrit H J Kema
Apr 7, 2016·Phytopathology·Hesham A Y GibrielMichael F Seidl
Oct 25, 2016·Molecular Ecology·Daniel Croll, Bruce A McDonald
May 17, 2018·Annual Review of Phytopathology·Andrea Sánchez-ValletDaniel Croll
Jan 27, 2019·Ecology and Evolution·Janine HaueisenEva H Stukenbrock
Feb 4, 2016·G3 : Genes - Genomes - Genetics·Megan C McDonaldPeter S Solomon
Jan 24, 2020·Frontiers in Plant Science·Xin MaJavier Palma-Guerrero
Sep 25, 2020·Fungal biology and biotechnology·Erin H Hill, Peter S Solomon
Dec 18, 2020·Frontiers in Plant Science·Guixia HaoMartha M Vaughan
Oct 24, 2020·Journal of Experimental Botany·Sujit Jung KarkiAngela Feechan
Dec 17, 2020·Fungal Genetics and Biology : FG & B·Elena FantozziGero Steinberg

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