Effector responses of bovine blood neutrophils against Escherichia coli: Role of NOD1/NF-κB signalling pathway

Veterinary Immunology and Immunopathology
Xun TanXu-Ping Yu

Abstract

Neutrophils use a broad array of pattern recognition receptors to sense and respond to invading pathogens and are important in the early control of acute bacterial infections. Nucleotide-binding oligomerizing domain-1 (NOD1) is a cytoplasmic receptor involved in recognizing bacterial peptidoglycan. Reduced neutrophil NOD1 expression has been reported in periparturient dairy cows. The aim of this study was to investigate the role of NOD1 signalling in the early responses of bovine neutrophils to bacterial infections. Blood neutrophils from healthy heifers were preincubated for 2h with ML130, a selective inhibitor of NOD1-dependent nuclear factor-κB (NF-κB) activation. Thereafter, cells were cultured with live Escherichia coli for additional 30 min or subjected to Boyden chamber cell migration assay with E. coli in the lower chamber. Results showed that ML130 inhibited E. coli-induced NF-κB nuclear translocation. There was an indication, although not significant, that ML130 down-regulated gene expression of proinflammatory cytokines interleukin (IL)-1β and tumour necrosis factor (TNF)-α, chemokines IL-8 and C-X-C motif ligand 2 (CXCL2), and adhesion molecule CD62L, in E. coli-challenged neutrophils. Flow cytometry-based Annexin V...Continue Reading

References

May 18, 1999·The Journal of Biological Chemistry·N InoharaG Núñez
Oct 3, 1999·Veterinary Immunology and Immunopathology·K Van OostveldtE Van den Eeckhout
Dec 23, 1999·Current Opinion in Hematology·P E NewburgerS M Weissman
Dec 4, 2001·Veterinary Immunology and Immunopathology·P S WeberJ L Burton
Jan 16, 2003·The Journal of Biological Chemistry·Stephen E GirardinPhilippe J Sansonetti
Jun 7, 2003·Science·Stephen E GirardinDana J Philpott
Jun 28, 2003·Blood·Fumitaka HayashiAndrew D Luster
Nov 25, 2003·Journal of Leukocyte Biology·Xueqing ZhangPeter E Newburger
Sep 1, 2004·American Journal of Veterinary Research·Araceli Diez-FrailleChristian Burvenich
Feb 25, 2005·Journal of Leukocyte Biology·Lisa C ParkerIan Sabroe
Mar 18, 2005·Annual Review of Immunology·Anthony W Segal
Jan 19, 2006·The Journal of Experimental Medicine·Junya MasumotoNaohiro Inohara
May 4, 2007·Cellular Microbiology·Adam J RatnerJeffrey N Weiser
Oct 13, 2007·European Journal of Clinical Investigation·K HocheggerM Rudnicki
Dec 15, 2007·The EMBO Journal·Mizuho HasegawaNaohiro Inohara
Oct 22, 2008·Annual Review of Pathology·Grace ChenGabriel Nuñez
Jan 6, 2009·Immunological Reviews·Luigi FranchiGabriel Nuñez
Jan 19, 2010·Nature Medicine·Thomas B ClarkeJeffrey N Weiser
Jan 19, 2011·Cell Research·Matthew S Hayden, Sankar Ghosh
Jul 12, 2011·Current Opinion in Pharmacology·Lynne R PrinceLisa C Parker
Jul 26, 2011·Nature Reviews. Immunology·Alberto MantovaniSébastien Jaillon
Sep 3, 2011·Immunological Reviews·Thomas B Clarke, Jeffrey N Weiser
Oct 18, 2011·ACS Medicinal Chemistry Letters·Pasha M KhanGregory P Roth
Aug 4, 2012·Veterinary Immunology and Immunopathology·Mieke G H StevensChristian Burvenich
Oct 2, 2012·Veterinary Immunology and Immunopathology·Xun TanJi-Yong Zhou
Nov 20, 2012·Frontiers in Immunology·Lilian O Moreira, Dario S Zamboni
Apr 2, 2013·Trends in Immunology·Christina J Thomas, Kate Schroder
Jan 1, 2013·Cells·Driss El Kebir, János G Filep

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Auditory Perception

Auditory perception is the ability to receive and interpret information attained by the ears. Here is the latest research on factors and underlying mechanisms that influence auditory perception.

Bacterial Pneumonia (ASM)

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Bacterial Pneumonia

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.