Effector role of cytochrome P450 reductase for androstenedione binding to human aromatase.

International Journal of Biological Macromolecules
Chao ZhangGianfranco Gilardi

Abstract

Cytochromes P450 constitute a large superfamily of monooxygenases involved in many metabolic pathways. Most of them are not self-sufficient and need a reductase protein to provide the electrons necessary for catalysis. It was shown that the redox partner plays a role in the modulation of the structure and function of some bacterial P450 enzymes. Here, the effect of NADPH-cytochrome reductase (CPR) on human aromatase (Aro) is studied for what concerns its role in substrate binding. Pre-steady-state kinetic experiments indicate that both the substrate binding rates and the percentage of spin shift detected for aromatase are increased when CPR is present. Moreover, aromatase binds the substrate through a conformational selection mechanism, suggesting a possible effector role of CPR. The thermodynamic parameters for the formation of the CPR-Aro complex were studied by isothermal titration calorimetry. The dissociation constant of the complex formation is 4.5 folds lower for substrate-free compared to the substrate-bound enzyme. The enthalpy change observed when the CPR-Aro complex forms in the absence of the substrate are higher than in its presence, indicating that more interactions are formed/broken in the former case. Taken toge...Continue Reading

References

Jan 6, 1992·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S D Black
Aug 5, 1997·Proceedings of the National Academy of Sciences of the United States of America·M WangJ J Kim
Nov 23, 2006·The Journal of Steroid Biochemistry and Molecular Biology·A BrodieD Jelovac
Dec 1, 2009·The Journal of Steroid Biochemistry and Molecular Biology·Yanyan HongShiuan Chen
Apr 14, 2010·The Journal of Biological Chemistry·Christal D Sohl, F Peter Guengerich
Mar 23, 2011·Steroids·Yanyan HongShiuan Chen
Sep 7, 2012·Journal of Medicinal Chemistry·Debashis GhoshHuw M L Davies
Apr 17, 2013·Biotechnology and Applied Biochemistry·Giovanna Di Nardo, Gianfranco Gilardi
Apr 17, 2013·Biotechnology and Applied Biochemistry·Xiaofeng YuRebecca C Wade
May 28, 2013·The Journal of Biological Chemistry·Shivani AhujaAyyalusamy Ramamoorthy
Aug 6, 2013·Structure·Wei-Cheng HuangGordon C K Roberts
Feb 14, 2014·Journal of the American Chemical Society·Wei ZhangShengying Li
Apr 22, 2014·Biophysical Chemistry·Stefano GianniPer Jemth
Nov 27, 2014·The Journal of Biological Chemistry·Giovanna Di NardoGianfranco Gilardi
Jul 22, 2016·Proceedings of the National Academy of Sciences of the United States of America·Scott A HollingsworthThomas L Poulos
Oct 6, 2016·The Journal of Steroid Biochemistry and Molecular Biology·Roberta BaravalleGianfranco Gilardi
Apr 20, 2017·The Journal of Physical Chemistry Letters·Alessandra MagistratoAndrea Cavalli
May 23, 2017·F1000Research·Carlo BarnabaAyyalusamy Ramamoorthy
Jun 5, 2017·Biochimica Et Biophysica Acta. Proteins and Proteomics·Roberta BaravalleGianfranco Gilardi
Sep 20, 2017·Biotechnology and Applied Biochemistry·Giovanna Di NardoGianfranco Gilardi
Feb 25, 2018·The Journal of Steroid Biochemistry and Molecular Biology·Debashis GhoshJessica Lo
Jun 5, 2018·Chemical Communications : Chem Comm·Carlo BarnabaAyyalusamy Ramamoorthy
Jul 12, 2018·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Carlo Barnaba, Ayyalusamy Ramamoorthy
May 2, 2019·Chemical Communications : Chem Comm·Mukesh MahajanAyyalusamy Ramamoorthy
May 11, 2019·The Journal of Biological Chemistry·F Peter GuengerichMichael J Reddish
May 31, 2019·The Journal of Biological Chemistry·F Peter GuengerichThanh T N Phan
Jan 28, 2020·The Journal of Physical Chemistry Letters·Ida RitaccoAlessandra Magistrato

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Citations

Aug 9, 2020·International Journal of Environmental Research and Public Health·Chao ZhangGiovanna Di Nardo
Jan 14, 2021·International Journal of Molecular Sciences·Giovanna Di NardoGianfranco Gilardi
Dec 19, 2020·Scientific Reports·Liang Xu, Liao Y Chen
Aug 31, 2021·RSC Chemical Biology·Samson A SouzaHo Leung Ng

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