Effects and interactions of low doses of arsenic and UVB on keratinocyte apoptosis

Chemical Research in Toxicology
Chih-Hung LeeHsin-Su Yu

Abstract

Although arsenic and ultraviolet light B (UVB) are both causes for skin cancers, lesions of arsenic-induced Bowen's disease are often confined to sun-protected skin. UVB may play a modulatory role in skin carcinogenesis by arsenic. The purpose of this study was to evaluate the effects and interactions of arsenic and UVB on cell cycle progression and apoptosis. Cultured human keratinocytes were treated with sodium arsenite (1 microM) and/or UVB (50 mJ/cm(2)) irradiation in different combinations: (i) arsenic alone, (ii) UVB alone, (iii) arsenic followed by UVB (As-UVB), and (iv) UVB followed by arsenic (UVB-As) treatments. Cell cycle analysis and BrdU pulsing revealed S phase arrest in all treatment groups and growth arrest in As-UVB and UVB-As groups. The terminal deoxynucleotidyl transferase-mediated deoxyuridine nick-end labeling assay showed a higher apoptosis rate in the UVB-As group as compared to that of the As-UVB and UVB groups. UVB irradiation significantly decreased Bcl-2 expression. In either the As-UVB or the UVB-As group, the expression of Bcl-2 was further suppressed as compared to the UVB group. The caspase-3, -8, and -9 relative activities were all increased in the UVB group; however, arsenic significantly enhan...Continue Reading

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Citations

Jun 30, 2006·Journal of Biomedical Science·Hsin-Su YuChee-Yin Chai
Nov 3, 2012·Case Reports in Oncological Medicine·Pratik GahalautSandhya Chauhan
Apr 14, 2007·Environmental Health Perspectives·Denis GanycWilliam T Self
Jan 23, 2009·The Journal of Investigative Dermatology·Zhongfa LuRalf Paus
Feb 2, 2013·The Journal of Investigative Dermatology·Chih-Hung LeeHsin-Su Yu
Apr 1, 2006·Mutation Research·Soile Tapio, Bernd Grosche
Dec 17, 2011·Journal of Toxicology·Victor D MartinezWan L Lam
Apr 26, 2011·The American Journal of Pathology·Chih-Hung LeeHsin-Su Yu
Jul 19, 2011·Metallomics : Integrated Biometal Science·Erika MitchellBibudhendra Sarkar
Dec 7, 2013·Journal of Cosmetic Dermatology·Nehal Mohamed Zuel-FakkarDina Mohamed Hamed
May 22, 2020·JCI Insight·Hélène ChoquetEric Jorgenson
Nov 26, 2019·Regulatory Toxicology and Pharmacology : RTP·I Palma-LaraA Hernández-Zavala
Feb 11, 2021·International Journal of Molecular Sciences·Eszter FidrusÉva Remenyik

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