Effects and interactions of medium components on laccase from a marine-derived fungus using response surface methodology.

Marine Drugs
Donna D'Souza-TicloChandralata Raghukumar

Abstract

The effects of various synthetic medium components and their interactions with each other ultimately impact laccase production in fungi. This was studied using a laccase-hyper-producing marine-derived basidiomycete, Cerrena unicolor MTCC 5159. Inducible laccases were produced in the idiophase only after addition of an inducer such as CuSO(4). Concentration of carbon and nitrogen acted antagonistically with respect to laccase production. A combination of low nitrogen and high carbon concentration favored both biomass and laccase production. The most favorable combination resulted in 917 U L(-1) of laccase. After sufficient growth had occurred, addition of a surfactant such as Tween 80 positively impacted biomass and increased the laccase activity to around 1,300 U L(-1). Increasing the surface to volume ratio of the culture vessel further increased its activity to almost 2,000 U L(-1).

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Citations

Sep 29, 2011·Applied Biochemistry and Biotechnology·Hui-Ying ChenShan-Jing Yao
Feb 28, 2019·International Microbiology : the Official Journal of the Spanish Society for Microbiology·Shaza M ElhusseinyRania I Shebl
Apr 18, 2019·International Journal of Molecular Sciences·Saowanee WikeeEric Record

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