Abstract
To investigate the effects of berberine (Berb) on dexamethasone- (Dex-) induced injury of human tendon cells and its potential mechanism. CCK-8 assay was used to explore the appropriate concentration of Dex-induced injury of tendon cells and the doses of Berb attenuates Dex cytotoxicity; cell wound healing assay was used to detect the effects (P < 0.05) of Berb and Dex on the migration ability of tendon cells; flow cytometry was used to measure cell apoptosis; DCF DA fluorescent probe was used to measure the ROS activity of cells. Western blotting was used to detect the expression of phenotype related factors including smooth muscle actin α (SMA-α), type I collagen (Col I), col III, apoptosis-related factors, caspase-3, cleaved caspase-3, caspase-9, cleaved caspase-9, and PI3K/AKT. CCK-8 assay showed that 1-100 μM Dex significantly inhibited the proliferation of tendon cells in a concentration-dependent manner (P < 0.05), where the inhibitory effect of 100 μM Dex was most significant (P < 0.005), and the pretreatment of 150, 200 μM Berb could reverse those inhibitions (all P < 0.05). Compared with the control group, Dex significantly inhibited cell migration (P < 0.05), while Berb pretreatment could enhance cell migration (P < ...Continue Reading
References
Jul 1, 1991·Journal of Gerontology·D AmielJ S VandeBerg
Jul 8, 2000·Biomaterials·D S TorresM Spector
Oct 17, 2003·Clinics in Sports Medicine·Nicola MaffulliLouis C Almekinders
Oct 18, 2003·The Journal of Bone and Joint Surgery. American Volume·Margaret Wan Nar WongCavor Kai Ming Chan
Mar 4, 2006·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·T J MolloyG A C Murrell
May 8, 2009·Acta Orthopaedica·Margaret Wan Nar WongKwong Man Lee
Oct 30, 2010·Journal of Applied Physiology·Chung-Hsun ChangJong-Hwei Su Pang
Oct 25, 2011·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Hsiang-Ning ChangYun-Ming Yang
Feb 22, 2012·Clinics in Sports Medicine·Christian C SkjongSherwin S W Ho
Oct 1, 2013·Seminars in Arthritis and Rheumatism·Benjamin John Floyd DeanAndrew J Carr
Jul 4, 2015·World Journal of Methodology·Stasinopoulos Dimitrios
Nov 12, 2016·BMC Musculoskeletal Disorders·Christoph SpangLudvig J Backman
Feb 14, 2017·Biochimie·Raheleh ShakeriJamshid Davoodi
Mar 16, 2017·Drug Metabolism Reviews·Kun WangFeng Qiu
Mar 30, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Tao WangMing H Zheng
May 8, 2018·Phytotherapy Research : PTR·Xiaorui Lin, Nan Zhang
Sep 7, 2018·Frontiers in Pharmacology·Maria A NeagAnca D Buzoianu
Oct 23, 2018·Phytotherapy Research : PTR·Yuan LiangFaguang Jin
May 23, 2019·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Kyunghoon MinYong-Soo Choi
Aug 11, 2019·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Dai-Qiang LiuDa-Wei Ye