Effects and molecular mechanisms of the antidiabetic fraction of Acorus calamus L. on GLP-1 expression and secretion in vivo and in vitro

Journal of Ethnopharmacology
Yun-Xi LiuHao-Shu Wu

Abstract

The radix of Acorus calamus L. (AC) is widely used in diabetes therapies in traditional folk medicine from America and Indonesia, and we have previously reported that the ethyl acetate fraction of AC (ACE) acts as an antidiabetic through insulin sensitizing, insulin releasing and alpha-glucosidase inhibitory activities. The present study is designed to investigate the effects and molecular mechanisms of ACE on glucagon-like peptide-1 (GLP-1) expression and secretion related to its hypoglycemic effects. The hypoglycemic effect of ACE (100mg/kg, i.g.) was confirmed by testing blood glucose levels or via oral glucose tolerance test (OGTT) in streptozotocin (STZ) induced hyperglycemic mice, db/db diabetic mice and diet-induced obese (DIO) mice. Plasma insulin, GLP-1 levels and intestinal GLP-1 related gene expression were determined in STZ-induced and db/db diabetic mice. The in vitro effects of ACE (12.5μg/ml) on the expression and secretion of GLP-1 were detected in NCI-H716 intestinal L-cells, and the correlation between ACE and molecules in the Wnt signaling pathway was further explored. ACE (100mg/kg) significantly lowered fasting blood glucose in STZ-induced and db/db diabetic mice and improved the OGTT in DIO mice. Insulin r...Continue Reading

References

Sep 1, 1989·Diabetes Care·C J Bailey, C Day
Dec 12, 2002·Diabetes·Patrick E MacDonaldMichael B Wheeler
Feb 19, 2003·The Biochemical Journal·Gabriela da Silva XavierGuy A Rutter
Feb 12, 2004·American Journal of Physiology. Endocrinology and Metabolism·Isabelle LeclercGuy A Rutter
Feb 18, 2004·Diabetologia·T Vilsbøll, J J Holst
Sep 15, 2005·The Annals of Pharmacotherapy·Cathi E DennehyAmy E Miller
Mar 7, 2006·Cell Metabolism·Daniel J Drucker
Sep 27, 2008·Endocrinology·Gareth E LimPatricia L Brubaker
Dec 17, 2008·Pharmacological Reviews·Wook Kim, Josephine M Egan
May 12, 2009·Journal of Ethnopharmacology·Hao-Shu WuQiao-Jun He
Dec 1, 2009·Biochemical Pharmacology·Yunli YuGuangji Wang
Jul 6, 2010·Biochimica Et Biophysica Acta·Qiong LiuXu Shen
May 24, 2012·Expert Opinion on Drug Safety·Jacqueline Kung, Robert R Henry
Nov 9, 2012·Clinical and Experimental Nephrology·Mitsuyoshi NambaJun-ichiro Miyagawa
Dec 18, 2012·Expert Opinion on Drug Safety·Stephanie Aleskow SteinStephen N Davis
May 21, 2013·Cell Metabolism·Jonathan E Campbell, Daniel J Drucker
Sep 24, 2013·Diabetes Therapy : Research, Treatment and Education of Diabetes and Related Disorders·Jaime A Davidson
Nov 16, 2013·The Journal of Endocrinology·Mi-Hyun KimMoon-Kyu Lee

❮ Previous
Next ❯

Citations

Jul 28, 2016·Journal of Ethnopharmacology·Saravanan V SathasivampillaiMichael Heinrich
Feb 2, 2018·Journal of Basic and Clinical Physiology and Pharmacology·Amrita Devi KhwairakpamAjaikumar B Kunnumakkara
Oct 3, 2019·Biomolecules·Bahare SalehiJavad Sharifi-Rad
Jul 2, 2018·Cardiovascular Diabetology·Mariël F van SteeAlbert K Groen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

Cardiovascular Biology of GLP-1

Glucagon-like peptide 1 (GLP-1) plays a role in glucose metabolism, energy homeostasis, and inflammation suppression. GLP-1 receptor signaling has been shown to impact cardiovascular function. This feed focuses on the role of GLP-1 and GLP-1 receptor agonists on cardiovascular biology.

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Biomarkers for Type 2 Diabetes

Biomarkers can help understand chronic diseases and assist in risk prediction for prevention and early detection of diseases. Here is the latest research on biomarkers in type 2 diabetes, a disease in which the body is unable to produce or properly use insulin.

CV Disorders & Type 2 Diabetes

This feed focuses on the association of cardiovascular diseases in patients with type 2 diabetes.