PMID: 9436261Jan 1, 1997Paper

Effects in the Comet assay of storage conditions on human blood

Teratogenesis, Carcinogenesis, and Mutagenesis
D AndersonR Marcos

Abstract

The Comet assay is a rapid and sensitive method for analyzing single cells for DNA damage. Using human lymphocytes, the assay is particularly useful for human monitoring studies, as well as for in vitro genotoxicity testing of chemicals. In such studies, it is not always possible to collect and process matched samples on the same day as the blood is taken. It would be useful if some samples could be stored and examined at a different time, without loss of viability or other factors affecting responses. It is thus important to understand the effects of storage conditions on blood to be used in such studies and how exposure or treatment might modify such responses. In a joint study in two laboratories, blood was taken from various donors and stored under different conditions. It was examined on day 1 (day on which sample was taken) and days 2, 3, 4, 5, or 8 at room temperature, 4 degrees C, or -20 degrees C. Cells were treated after storage (from day 2 onward) with bleomycin (BLM) and ethylnitrosourea (ENU). The data were analyzed either by eye (classifying cells with different categories of damage) or by using a computerized image analysis system (Kinetic Imaging Ltd., Liverpool UK. Software Package Comet 3.0) where the tail mom...Continue Reading

Citations

Jun 3, 1998·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·P VillaniA Carere
Sep 2, 2008·International Seminars in Surgical Oncology : ISSO·Fraz A MalikRafshan Sadiq
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Nov 1, 2012·Indian Journal of Occupational and Environmental Medicine·Ajanta Halder, Madhusnata De
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Feb 17, 2017·Environmental and Molecular Mutagenesis·Khaled HabasDiana Anderson

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