PMID: 25784992Mar 19, 2015Paper

Effects of α-enolase (ENO1) over-expression on malignant biological behaviors of AGS cells

International Journal of Clinical and Experimental Medicine
Yang-Qi LiuQi-Lian Liang

Abstract

To investigate the effects of α-Enolase (ENO1) over-expression on the proliferative and migratory abilities of AGS cells. The target gene was cloned and mounted to the eukaryotic expression vector pcDNA3.1(+), then was transfected into gastric cancer cell lines AGS. mRNA and protein level of ENO1 in AGS cells were verified by real-time quantitative RT-PCR and Western Blot, respectively. The effects of over-expression of ENO1 on proliferative and migratory abilities of AGS cells were detected by the experiments of CCK-8, colony formation and wound healing assays. The eukaryotic expression vector pcDNA3.1(+)/eno1 was successfully constructed, and verified by sequencing. It was shown from the cell proliferation curves that the proliferative ability of AGS-ENO1 transfected group was higher than that of the control group after 72 hours (t = 3.44, P = 0.04), meanwhile, the number of the cell-colonies of the AGS-ENO1 group were significantly greater than that of the control group (t = 5.26, P = 0.01). For the ability of migration, it was significantly enhanced in the over-expression ENO1 cells than in the negative cells (t = 7.35, P < 0.001). The over-expression of ENO1 protein can enhance the abilities of proliferation and migration ...Continue Reading

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