Effects of β-Sitosterol from Corn Silk on TGF-β1-Induced Epithelial-Mesenchymal Transition in Lung Alveolar Epithelial Cells

Journal of Agricultural and Food Chemistry
Yong Joo ParkKyu Hyuck Chung

Abstract

Pulmonary fibrosis is a chronic lung disease characterized by abnormal accumulation of the extracellular matrix (ECM). Chronic damage of the alveolar epithelium leads to a process called "epithelial-mesenchymal transition" (EMT) and increases synthesis and deposition of ECM proteins. Therefore, inhibition of EMT might be a promising therapeutic approach for the treatment of pulmonary fibrosis. β-Sitosterol is one of the most abundant phytosterols in the plant kingdom and the major constituent in corn silk, which is derived from the stigma and style of maize (Zea mays). In this study, we elucidated that β-sitosterol inhibited transforming growth factor-β1 (TGF-β1)-induced EMT and consequently had an antifibrotic effect. β-Sitosterol (1-10 μg/mL) significantly downregulated the TGF-β1-induced fibrotic proteins, such as collagen, fibronectin, and α-smooth muscle actin in human alveolar epithelial cells (p < 0.01). After 24 h, relative wound density (RWD) was increased in TGF-β1 treated group (82.16 ± 5.70) compare to the control group (64.63 ± 2.21), but RWD was decreased in β-sitosterol cotreated group (10 μg/mL: 71.54 ± 7.39; 20 μg/mL: 65.69 ± 6.42). In addition, the changes of the TGF-β1-induced morphological shape and protein ...Continue Reading

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Citations

Sep 4, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Shyamaladevi Babu, Selvaraj Jayaraman
Jul 20, 2021·European Journal of Pharmacology·Qihao LiYu Ou
Nov 6, 2021·BMC Complementary Medicine and Therapies·Suranat Phonghanpot, Faongchat Jarintanan
Oct 15, 2021·Journal of Oleo Science·Beibei ZhaoZhicheng Chen

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