Effects of 10-hydroxycamptothecin on intrinsic mitochondrial pathway in silkworm BmN-SWU1 cells

Pesticide Biochemistry and Physiology
Chun PanCheng Lu

Abstract

10-Hydroxycamptothecin (HCPT), a plant alkaloid isolated from Camptotheca acuminate, is known as a planted-derived insecticide, however, the specific mechanism in insect cells is still unclear. In this study, we treated the ovarian cell line of the silkworm, BmN-SWU1, with different HCPT doses for durations ranging from 0 to 72h. The apoptosis morphology was evident after 72h of incubation and included cell protuberance, concentrated cytoplasm and apoptotic bodies. We observed DNA fragmentation and cell apoptosis after HCPT treatment. The disruption of mitochondrial distribution, activation of the intracellular mitochondrial permeability transition pore, and release of cytochrome c during HCPT-induced apoptosis in dose and time-dependent manner indicate the involvement of mitochondria in BmN-SWU1 cells. Caspase-9 and -3 activities increased gradually with the duration of incubation time. In conclusion, HCPT has a significant effect to initiate the intrinsic mitochondrial pathway in silkworm cells, providing a theoretical basis for better application of plant-derived insecticide in pest control.

References

Sep 1, 1996·The Journal of Experimental Medicine·P MarchettiG Kroemer
Apr 1, 1996·Journal of Bioenergetics and Biomembranes·P Bernardi, V Petronilli
May 2, 1998·The EMBO Journal·C ScaffidiM E Peter
Aug 28, 1998·Science·A Ashkenazi, V M Dixit
Mar 26, 2003·Nature Cell Biology·Motoshi SawadaShigemi Matsuyama
Feb 24, 2005·Cancer Biology & Therapy·Zhaoyu Jin, Wafik S El-Deiry
Mar 23, 2007·Nature Reviews. Molecular Cell Biology·Stefan J Riedl, Guy S Salvesen
May 10, 2007·Developmental Cell·Eltyeb AbdelwahidKristin White

❮ Previous
Next ❯

Citations

Sep 24, 2020·The Journal of General Physiology·Jasmine A NirodyPadmini Rangamani
Mar 15, 2018·International Journal of Molecular Sciences·Jingjing ZhangGuohua Zhong
Jan 13, 2021·Pest Management Science·Jin-Yan WangNian-Feng Wan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis