Effects of 100 years wastewater irrigation on resistance genes, class 1 integrons and IncP-1 plasmids in Mexican soil

Frontiers in Microbiology
Sven JechalkeElisabeth Grohmann

Abstract

Long-term irrigation with untreated wastewater can lead to an accumulation of antibiotic substances and antibiotic resistance genes in soil. However, little is known so far about effects of wastewater, applied for decades, on the abundance of IncP-1 plasmids and class 1 integrons which may contribute to the accumulation and spread of resistance genes in the environment, and their correlation with heavy metal concentrations. Therefore, a chronosequence of soils that were irrigated with wastewater from 0 to 100 years was sampled in the Mezquital Valley in Mexico in the dry season. The total community DNA was extracted and the absolute and relative abundance (relative to 16S rRNA genes) of antibiotic resistance genes (tet(W), tet(Q), aadA), class 1 integrons (intI1), quaternary ammonium compound resistance genes (qacE+qacEΔ1) and IncP-1 plasmids (korB) were quantified by real-time PCR. Except for intI1 and qacE+qacEΔ1 the abundances of selected genes were below the detection limit in non-irrigated soil. Confirming the results of a previous study, the absolute abundance of 16S rRNA genes in the samples increased significantly over time (linear regression model, p < 0.05) suggesting an increase in bacterial biomass due to repeated i...Continue Reading

References

Oct 31, 2000·Applied and Environmental Microbiology·M T SuzukiE F DeLong
Dec 3, 2004·Applied and Environmental Microbiology·Marilyn S SmithDavid W Graham
Jun 15, 2005·Environmental Science & Technology·Ramunas StepanauskasJ V McArthur
Mar 8, 2006·Environmental Toxicology and Chemistry·Chad A KinneyJeffery D Cahill
Mar 16, 2006·Trends in Microbiology·Craig Baker-AustinJ V McArthur
Jan 24, 2007·Water Research·Eric A AuerbachKatherine D McMahon
Oct 5, 2007·The Journal of Antimicrobial Chemotherapy·Alexandra MouraAntónio Correia
Jun 7, 2008·Current Opinion in Biotechnology·Fernando BaqueroRafael Cantón
Mar 3, 2009·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·R Cantón
Jul 8, 2009·Research in Microbiology·Chu T T BinhKornelia Smalla
Jun 15, 2010·The Journal of Antimicrobial Chemotherapy·O BarraudM C Ploy
Sep 21, 2010·Current Opinion in Microbiology·Gerard D Wright
Sep 29, 2011·Applied and Environmental Microbiology·Diya SenEva M Top
Apr 18, 2012·Frontiers in Microbiology·Thibault StalderMarie-Cécile Ploy
Dec 18, 2012·Applied and Environmental Microbiology·Sven JechalkeHolger Heuer
Jan 26, 2013·The Lancet Infectious Diseases·Elizabeth M H WellingtonA Prysor Williams
Feb 5, 2013·Environmental Science and Pollution Research International·Joao Gatica, Eddie Cytryn
Feb 19, 2013·Ecotoxicology and Environmental Safety·Chryssa BoukiEvan Diamadopoulos
Mar 9, 2013·Frontiers in Microbiology·Magdalena Popowska, Agata Krawczyk-Balska
Apr 9, 2013·Journal of Environmental Management·Diana Norton-BrandãoJules B van Lier
Dec 1, 2009·Environmental Microbiology Reports·F BaqueroJ L Martinez
Sep 13, 2013·Chemosphere·José Rivera-UtrillaRaúl Ocampo-Pérez

❮ Previous
Next ❯

Citations

Mar 16, 2018·EcoHealth·Lisa M Durso, Kimberly L Cook
Mar 6, 2019·MSystems·Taylor K DunivinAshley Shade
Oct 30, 2020·Environment International·Ioannis D KampourisThomas U Berendonk

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR

Software Mentioned

SAS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.