Effects of 1,1,1-trichloro-2,2-bis(p-chlorophenyl)-ethane (p,p'-DDT) on 3T3-L1 and 3T3-F442A adipocyte differentiation

Biochemical Pharmacology
Maria J Moreno-Aliaga, Fumio Matsumura

Abstract

Based upon our initial observations that 1,1,1-trichloro-2,2-bis(p-chlorophenyl)-ethane (p,p'-DDT) induces a concentration-dependent increase in 3T3-L1 adipocyte differentiation, the mechanism of the p,p'-DDT-induced adipocyte differentiation was studied, using 3T3-L1 and 3T3-F442A cells. Since, it is known that the differentiation of the 3T3-L1 adipocyte cell line involves the induction of the transcription factors CCAAT enhancer binding protein beta (C/EBPbeta), peroxisome proliferator-activated receptor gamma (PPARgamma), and C/EBPalpha, the possible role of these factors in p,p'-DDT-induced adipocyte differentiation had to be examined. It was found that p,p'-DDT-treated 3T3-L1 cells showed a concentration-dependent increase in the nuclear levels of both PPARgamma and C/EBPalpha protein. On the other hand, treatment with p,p'-DDT (20 microM) did not affect the expression pattern of C/EBPbeta protein during differentiation. Gel shift analysis of nuclear proteins for binding to the C/EBP recognition site of DNA showed an increase in binding activity at day 2 of differentiation in p,p'-DDT-treated cells. Supershift analysis revealed that this rise was caused mainly by a dramatic increase in the abundance of the C/EBPalpha-DNA c...Continue Reading

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Citations

Feb 6, 2013·Environmental Health and Preventive Medicine·Hidekuni Inadera
Apr 5, 2011·Journal of Physiology and Biochemistry·Marta Fernández-GalileaMaría Jesús Moreno-Aliaga
Dec 12, 2012·Environmental Health Perspectives·Michele La MerrillRobert Barouki
Mar 21, 2013·Environmental Health Perspectives·Marcella WarnerBrenda Eskenazi
Apr 12, 2014·American Journal of Epidemiology·Marcella WarnerBrenda Eskenazi
Nov 9, 2010·Annual Review of Physiology·Cristina Casals-Casas, Béatrice Desvergne
Apr 19, 2011·The Journal of Nutritional Biochemistry·Silvia Lorente-CebriánMaria J Moreno-Aliaga
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Mar 31, 2010·Molecular Nutrition & Food Research·Silvia Lorente-CebriánMaría Jesús Moreno-Aliaga
Aug 19, 2011·Drug and Chemical Toxicology·Eun Jee LeeByung Sun Yoo
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Feb 4, 2014·Endocrine Reviews·Duk-Hee LeeLaura N Vandenberg
Sep 15, 2019·Environmental Toxicology and Pharmacology·Lesa A ThompsonMayumi Ishizuka

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