Effects of 1,2-propanediol and freezing-thawing on the in vitro developmental capacity of human immature oocytes

Fertility and Sterility
W Y SonK Y Cha

Abstract

To investigate effects of 1,2-propanediol and freezing-thawing treatment on the maturation and developmental capacity of the human immature oocytes obtained from unstimulated ovaries. Intact cumulus-enclosed immature oocytes collected from unstimulated ovaries were divided into three groups, such as no treatment as control (group 1), only 1,2-propanediol-treated (group 2), and cryopreserved group (group 3). Oocytes in group 1, group 2, and survived oocytes from cryopreservation in group 3 were cultured for 48 hours. A random selection of matured oocytes was inseminated with normal donor sperm to evaluate the fertilization and developmental capacity. Infertility Medical Center at the CHA General Hospital, Seoul, Korea. Oocytes were obtained from patients undergoing gynecological surgery. Rates of survival, maturation to metaphase II, fertilization, and cleavage. Survival rate after freezing-thawing in group 3 was 55.1% (54/98). Oocytes were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 20% fetal bovine serum, 10 IU/mL pregnant mare serum gonadotropin, and 10 IU/mL hCG. Maturation rates were 76.8% (63/82), 67.1% (47/70), and 59.3% (32/54) in the groups 1, 2, and 3, respectively. Maturation rate in group ...Continue Reading

Citations

Mar 8, 2005·The Journal of Reproduction and Development·Ki-Eun ParkKoji Niwa
Sep 12, 2007·Human Reproduction Update·Debra A Gook, David H Edgar
Dec 13, 2012·Future Oncology·Ana Luiza BerwangerMichael Grynberg
Feb 24, 2001·Hospital Medicine·O SalhaA Rutherford
Oct 12, 2013·Journal of Assisted Reproduction and Genetics·Fausta BrambillascaRubens Fadini
Nov 4, 2008·Obstetrics and Gynecology·Togas TulandiSeang Lin Tan
Aug 24, 2007·Reproduction, Fertility, and Development·A DinnyesT L Nedambale
Aug 6, 2019·Frontiers in Endocrinology·Weon-Young SonWilliam Buckett
Nov 18, 2011·Journal of Assisted Reproduction and Genetics·Catherine M H CombellesCatherine Racowsky
Apr 6, 2012·Journal of Assisted Reproduction and Genetics·Giovanna FasanoYvon Englert
Jan 13, 2001·Molecular and Cellular Endocrinology·K Y ChaT K Yoon
Jun 1, 2001·Fertility and Sterility·N A CekleniakC Racowsky
Mar 29, 2000·Theriogenology·J M ShawA O Trounson
Jun 22, 2006·Reproductive Toxicology·G AmbrosiniM Moscarini

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.