Effects of 16,16-dimethyl prostaglandin E2 and indomethacin on leukotriene B4 and inflammation in rabbit colitis

Prostaglandins
R SchumertR D Zipser

Abstract

The role of increased prostaglandin production and the effects of exogenous prostaglandins on inflammation of colitis are not established. We administered intramuscular 16,16-dimethyl prostaglandin E2 (DiM-PGE2) and indomethacin to rabbits with formalin immune-complex colitis and measured leukotriene B4 (LTB4), prostaglandin E2 (PGE2) and severity of inflammation. DiM-PGE2 (100 micrograms/kg/BID) reduced LTB4 production (from 401 +/- 108 to 216 +/- 58 pg/ml) and infiltration of neutrophils, mucosal necrosis, inflammatory exudate and edema (all P less than 0.05). Other studies determined that parenteral DiM-PGE2 did not reduce the initial chemical damage induced by formalin, suggesting that cytoprotection of chemical insult was not the mechanism of suppressed inflammation in the immune colitis model. Indomethacin (10 mg/kg/d) reduced endogenous PGE2 by 80%, but did not reduce leukotriene production or inflammation. Exogenous prostaglandins cause a dose-dependent suppression of inflammation in experimental colitis, by a mechanism other than cytoprotection of chemical-induced mucosal injury.

References

Sep 1, 1985·Digestive Diseases and Sciences·J L WallaceN K Boughton-Smith
Jun 1, 1985·Prostaglandins·A RobertM J Ruwart
Feb 1, 1981·Prostaglandins and Medicine·S R GouldJ E Lennard-Jones
May 1, 1981·Postgraduate Medical Journal·D S Rampton, G E Sladen
Oct 1, 1983·Journal of Clinical Immunology·J S Goodwin, J Ceuppens
Dec 1, 1984·Gut·D S Rampton, C J Hawkey
Jul 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·E A HamF A Kuehl
Jul 1, 1982·Clinical and Experimental Dermatology·R D Camp

Citations

Jun 1, 1990·Agents and Actions·L R FitzpatrickD L Decktor
Jul 1, 1995·Gastroenterology·C E Eberhart, R N Dubois
Feb 1, 1990·The Journal of Clinical Investigation·Fabio CominelliR D Zipser
Apr 1, 1990·Gastroenterology·L E LeDuc, C C Nast
Apr 1, 1992·Prostaglandins, Leukotrienes, and Essential Fatty Acids·K J TorsherR N Fedorak
Dec 1, 1990·Prostaglandins, Leukotrienes, and Essential Fatty Acids·D J FretlandT S Gaginella
Aug 10, 2007·Expert Opinion on Investigational Drugs·Laurence Guy HowesCatherine Walker

Related Concepts

16,16-dimethylprostaglandin E, (11alpha,13E,15S)-isomer
Metazoa
Colitis
Taenia Coli
Dose-Response Relationship, Drug
Osmosin
Leukotrienes B
Necrosis
Prostaglandins E, Synthetic
Chinchilla Rabbits

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