Effects of 17β-estradioland raloxifene on endothelial OPG and RANKL secretion

Ginekologia polska
Wojciech KarwowskiBeata Naumnik

Abstract

This study aims to asses the effects of estradiol vs. raloxifene on the levels of osteoprotegerin and soluble Receptor Activator of Nuclear Factor kB Ligand (sRANKL) in Human Umbilical Vein Endothelial Cells (HUVEC) culture in standard and calcifying medium. Human Umbilical Vein Endothelial Cells were isolated from human umbilical vein by standard method. The supernatant concentrations of osteoprotegerin (OPG) and sRANKL (ELISA) were determined after incubation with glicerophosphate, estradiol , raloxifene, glicerophoshate and estradiol, glicerophosphate and raloxifene in comparison with control group at four designated time points (0, 1, 2 and 4 days of incubation). Incubation of estradiol with HUVEC colony lowered the OPG level significantly after day 2 and 4. Meantime, the level of sRANKL was stable. Raloxifene added to standard growth medium also significantly lowered OPG concentration after day 4 only, with no impact on sRANKL concentration. When added to calcifying medium, both estradiol and raloxifene significantly changed OPG level during the experiment. In all treated groups OPG levels were lower than in groups exposed to calcifying medium only. Neither estradiol, nor raloxifene changed sRANKL levels during the experim...Continue Reading

Citations

Apr 17, 2020·International Journal of Molecular Sciences·Sun Joo LeeJae-Han Jeon
Mar 20, 2019·Applied Microbiology and Biotechnology·Manyu LuoHuili Lu
Jul 3, 2021·International Journal of Molecular Sciences·Giulia Chinetti, Jaap G Neels

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