PMID: 15226595Jul 1, 2004Paper

Effects of 17beta-estradiol on in vitro maturation of pig oocytes in protein-free medium

The Journal of Reproduction and Development
Qing LiM G Hunter

Abstract

The present study examined the effects of 17 beta-estradiol (E(2)) on in vitro maturation and subsequent in vitro fertilization of pig oocytes matured with or without cumulus cells. When E(2) (10 ng/ml) was added to the protein-free maturation medium, the proportions of cumulus-enclosed oocytes that underwent germinal vesicle breakdown and reached metaphase II were significantly reduced (P<0.05), and cumulus expansion was also significantly inhibited (P<0.05) compared with the control (no E(2) added). Although oocytes matured in the presence of E(2) were penetrated by sperm in vitro at the same level as the control, the incidences of male pronuclear (MPN) formation and activated oocytes were significantly lower (P<0.05) than the control. These inhibitory effects of E(2) were prevented when the medium was supplemented with E(2) together with its antagonist, ICI 182,780 (1 microg/ml), although the presence of the antagonist alone in the medium had no effect on the maturation and fertilization in vitro of oocytes. In cumulus-free oocytes, E(2) had no effect on nuclear maturation and penetration in vitro, but low MPN formation was observed in oocytes matured in the presence and absence of E(2). When cumulus-enclosed oocytes were cu...Continue Reading

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Citations

May 25, 2011·The Journal of Reproduction and Development·Rentsenkhand SambuuJohanna Fink-Gremmels
Jul 4, 2013·Journal of Veterinary Science·Jungmin SonJongki Cho
Dec 18, 2013·Acta Veterinaria Hungarica·Clara MaloNoelia Gonzalez
Jun 4, 2005·Human Reproduction·Suhail A R DoiKamal A S Al-Shoumer

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