Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on fetal mouse urinary tract epithelium in vitro

Toxicology
P L BryantB D Abbott

Abstract

2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), produces hydronephrosis by altering the differentiation and proliferation of ureteric epithelial cells in the fetal C57BL/6N mouse urinary tract. This study tests the hypothesis that the late fetal urinary tract epithelial cells respond to TCDD with increased proliferation and that the responses do not require contributions from other maternal or fetal tissues. This was achieved by exposing late gestation fetal urinary tract cells to TCDD in an in vitro model. Isolated ureteric cells from gestation day (GD) 18 fetal ureters were plated in medium supplemented with trace elements, a complex mixture of lipids, a defined mixture of purified hormones and growth factors. Both epithelial and mesenchymal cells remain viable under these conditions. The cultures were exposed to 0.1% dimethylsulfoxide (DMSO), 1x10(-8), 1x10(-9) or 1x10(-10) M TCDD. Exposure to 1x10(-10) M TCDD did not affect the cultures, while 1x10(-8) and 1x10(-9) M TCDD supported epithelial, but not mesenchymal, cell survival and stimulated epithelial cell proliferation and differentiation. The TCDD-exposed cells expressed high levels of keratin and little or no vimentin, confirming that the cells, which survive and different...Continue Reading

References

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Citations

Dec 8, 2004·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Ho Jae HanJang Hern Lee
Nov 7, 2009·European Journal of Epidemiology·Anh Duc NgoChristine L Roberts
Mar 18, 2006·International Journal of Epidemiology·Anh D NgoTuan V Nguyen
Nov 5, 2002·Journal of Exposure Analysis and Environmental Epidemiology·Arslan AkhmedkhanovPaolo Toniolo
Dec 8, 2005·Birth Defects Research. Part A, Clinical and Molecular Teratology·Sara A CarneyRichard E Peterson

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