PMID: 641378May 1, 1978Paper

Effects of 4-tertiary butyl catechol on tissue cultured melanocytes

The Journal of Investigative Dermatology
J D MansurW L Epstein

Abstract

Depigmenting effects of 4-tertiary butyl catechol (TBC) were investigated using tissue cultured melanocytes of adult guinea pig. Black guinea pig ear epidermis was trypsinized, suspended in BME Eagle media (5 X 10(6) cells) supplemented with 10% calf serum, and seeded in Petri dishes (2 ml/ea). On the 5th day of culture, TBC dissolved in DMSO was added to the media, and the central area of Petri dishes with large numbers of melanocytes (but not keratinocytes) present were examined by light and electron microscopy. After 6 hr of treatment with 1.5 X 10(-3) mg TBC per ml of media, about 15 to 30% of melanocytes detached and about half of the remaining cells showed reversible changes in the cell shapes. An increase of TBC concentration to more than 2 X 10(-3) mg per ml of media resulted in larger numbers of melanocyte deaths. The surviving cells contained numerous, but ultrastructurally unusual premelanosomes and melanosomes. In addition, microfilaments showed a wavy appearance.

Citations

Jul 1, 1979·Contact Dermatitis·G A GellinM Ring
May 1, 1982·Contact Dermatitis·B E Björkner
Jun 1, 1981·The British Journal of Dermatology·S Passi, M Nazzaro-Porro
May 1, 1980·Journal of Toxicology and Environmental Health·Y UsamiG A Gellin
Nov 22, 2018·Environmental Toxicology·Gopalapura J VishalakshiKesturu S Girish
Mar 1, 1984·Clinical and Experimental Dermatology·K E Sharquie
Jan 1, 1984·The Journal of Investigative Dermatology·T KawashimaK Fukuyama

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