Effects of 5-Aminolevulinic Acid on Gene Expression, Immunity, and ATP Levels in Pacific White Shrimp, Litopenaeus vannamei

Marine Biotechnology
Ivane R Pedrosa-GerasmioIkuo Hirono

Abstract

With the emergence of several infectious diseases in shrimp aquaculture, there is a growing interest in the use of feed additives to enhance shrimp immunity. Recently, the use of 5-aminolevulinic acid (5-ALA), a non-protein amino acid that plays a rate-limiting role in heme biosynthesis, has received attention for its positive effect on immunity in livestock animals. To evaluate the effect of 5-ALA in the Pacific white shrimp, Litopenaeus vannamei, we conducted microarray analysis, a Vibrio parahaemolyticus immersion challenge test, an ATP level assay, and gene expression analysis of some hemoproteins and genes associated with heme synthesis and degradation. Out of 15,745 L. vannamei putative genes on the microarray, 101 genes were differentially expressed by more than fourfold (p < 0.05) between 5-ALA-supplemented and control shrimp hepatopancreas. 5-ALA upregulated 99 of the 101 genes, 41 of which were immune- and defense-related genes based on sequence homology. Compared to the control, the 5-ALA-supplemented group had a higher survival rate in the challenge test, higher transcript levels of porphobilinogen synthase, ferrochelatase, catalase, nuclear receptor E75, and heme oxygenase-1 and higher levels of ATP. These findings...Continue Reading

References

Dec 1, 1989·Trends in Biochemical Sciences·G PadmanabanP N Rangarajan
Apr 10, 1999·Insect Biochemistry and Molecular Biology·R L TellamP Willadsen
Feb 8, 2002·Applied Microbiology and Biotechnology·K SasakiT Tanaka
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Nov 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·David E BarananoSolomon H Snyder
Jul 3, 2004·Ageing Research Reviews·Hani Atamna
Mar 4, 2006·Pharmacology & Therapeutics·Asterios S TsiftsoglouLefkothea C Papadopoulou
Jun 7, 2008·Current Opinion in Biotechnology·Fernando BaqueroRafael Cantón
Feb 25, 2009·Nucleic Acids Research·J M RuijterA F M Moorman
May 19, 2009·Developmental and Comparative Immunology·Xian-Wei WangJin-Xing Wang
Jul 4, 2009·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Wei-Na WangAn-Li Wang
Dec 23, 2009·Fish & Shellfish Immunology·Cui-Luan YaoJian-Hai Xiang
Feb 18, 2010·Marine Drugs·Yong ZhaoRiccardo A A Muzzarelli
Mar 30, 2010·Insect Biochemistry and Molecular Biology·Bernard Moussian
May 28, 2010·Protein Science : a Publication of the Protein Society·Gunhild LayerDirk W Heinz
Nov 26, 2010·Molecular Plant-microbe Interactions : MPMI·S T LillyR M MacDiarmid
Dec 15, 2010·International Immunopharmacology·Masahiro IshizukaTohru Tanaka
Mar 19, 2011·BMC Research Notes·Shun-Ichiro OguraTohru Tanaka
Mar 25, 2011·Methods in Molecular Biology·Mohan C VemuriMahendra S Rao
Jul 1, 2011·Genes & Development·Lucía CáceresHenry M Krause
Feb 2, 2012·European Journal of Cell Biology·Khaleelulla Saheb ShaikBernard Moussian
Mar 21, 2012·Journal of Invertebrate Pathology·Timothy W Flegel
Jun 26, 2012·Nucleic Acids Research·Andreas UntergasserSteven G Rozen
Oct 13, 2012·Fish & Shellfish Immunology·Anchalee TassanakajonSureerat Tang
Nov 13, 2012·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Carlos H Trasviña-ArenasGloria Yepiz-Plascencia
Jan 4, 2013·PloS One·Rungnapa LeelatanawitNitsara Karoonuthaisiri
Feb 18, 2014·International Immunopharmacology·Yoshiaki NishioXiao-Kang Li
Nov 6, 2014·PloS One·Wankun DengYu Xue

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Methods Mentioned

BETA
PCR
Feature Extraction
environmental stress

Software Mentioned

Agilent Feature Extraction
BLASTX
Blast2GO
Heatmap Illustrator
GraphPad Prism
LinRegPCR
Subio Platform

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