DOI: 10.1101/476069Nov 22, 2018Paper

Effects of 5-aza-2 ́-deoxycytidine on human osteoarthritic chondrocytes

BioRxiv : the Preprint Server for Biology
Shirin KadlerMark Rosowski

Abstract

Background: Given regenerative therapies, the utilization of primary human cells is desired and requested in the development of in vitro systems and disease models. After a few passages in vitro, all cells from the connective tissue end up in a similar fibroblastoid cell type marked by loss of the specific expression pattern. It is still under discussion whether different de-differentiated mesenchymal cells have similar or identical differentiation capacities in vitro. Methods: Chondrocytes isolated from patients with late-stage osteoarthritis were cultured for several passages until de-differentiation was completed. The mRNA level of cartilage markers was investigated, and the adipogenic, osteogenic and chondrogenic differentiation capacity was examined. By adding 5-aza-2′-deoxycytidine (5-aza-dC) to the media, the influence of DNA methylation on the differentiation capacity was analyzed. Results: The chondrocytes used in this work were not affected by the loss of specific gene expression upon cell culture. The mRNA levels of SOX5, SOX6, SOX9, aggrecan, and proteoglycan-4 remained unchanged. The underlying mechanisms of cartilage marker maintenance in osteoarthritic (OA) chondrocytes were investigated with a focus on the epige...Continue Reading

Related Concepts

Azacitidine
Biological Markers
Cartilage
Cell Culture Techniques
Cell Differentiation Process
Connective Tissue
Gene Expression
Kidney Failure, Chronic
Osteogenesis
Proteoglycan

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