Effects of 5α-cholestan-3-one on the synaptic vesicle cycle at the mouse neuromuscular junction

Biochimica Et Biophysica Acta
M R KasimovA M Petrov

Abstract

We have investigated the effects of 5α-cholesten-3-one (5Ch3, 200 nM) on synaptic transmission in mouse diaphragm. 5Ch3 had no impact on the amplitude or frequency of miniature endplate currents (MEPCs, spontaneous secretion), but decreased the amplitude of EPCs (evoked secretion) triggered by single action potentials. Treatment with 5Ch3 increased the depression of EPC amplitude and slowed the unloading of the dye FM1-43 from synaptic vesicles (exocytosis rate) during high-frequency stimulation. The estimated recycling time of vesicles did not change, suggesting that the decline of synaptic efficiency was due to the reduction in the size of the population of vesicles involved in release. The effects of 5Ch3 on synaptic transmission may be related to changes in the phase properties of the membrane. We have found that 5Ch3 reduces the staining of synaptic regions with the B-subunit of cholera toxin (a marker of lipid rafts) and increases the fluorescence of 22-NBD-cholesterol, indicating a phase change within the membrane. Manipulations of membrane cholesterol (saturation or depletion) strongly reduced the influence of 5Ch3 on both FM1-43 dye unloading and staining with the B-subunit of cholera toxin. Thus, 5Ch3 reduces the numb...Continue Reading

References

Apr 5, 2001·Biochimica Et Biophysica Acta·L M LouraM Prieto
May 3, 2003·The EMBO Journal·Christopher J R LoewenTimothy P Levine
Aug 5, 2003·Neuron·David A RichardsWilliam J Betz
Mar 9, 2004·Neuron·Ruth ReaRichard H Kramer
Jun 26, 2004·Annual Review of Neuroscience·Thomas C Sudhof
Dec 22, 2004·Nature Reviews. Neuroscience·Silvio O Rizzoli, William J Betz
Dec 13, 2005·The Journal of Physiology·Orit Zamir, Milton P Charlton
Feb 28, 2007·Immunology and Cell Biology·Norbert BlankHanns-Martin Lorenz
Dec 5, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Aleksej M PetrovAndrej L Zefirov
May 12, 2009·Nature Neuroscience·Naila Ben Fredj, Juan Burrone
Feb 22, 2011·Current Opinion in Neurobiology·Denise M O Ramirez, Ege T Kavalali
Mar 23, 2011·Chemistry and Physics of Lipids·Terhi VihervaaraVesa M Olkkonen
Apr 26, 2011·Chemistry and Physics of Lipids·John J Mackrill
Apr 26, 2011·Chemistry and Physics of Lipids·Valerio Leoni, Claudio Caccia
Jun 2, 2011·Cold Spring Harbor Perspectives in Biology·Kai Simons, Julio L Sampaio
Jun 30, 2012·Cold Spring Harbor Perspectives in Biology·AbdulRasheed A Alabi, Richard W Tsien
Jul 24, 2012·Neuropharmacology·Ana M SebastiãoJoaquim A Ribeiro
Feb 23, 2013·Biochemical Pharmacology·Marion Weber-BoyvatVesa M Olkkonen
Jun 13, 2013·Trends in Cell Biology·Dmytro Puchkov, Volker Haucke
Jun 14, 2013·Current Opinion in Lipidology·Ingemar Björkhem
Nov 1, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Steven M PaulCharles F Zorumski

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Citations

Mar 10, 2017·American Journal of Physiology. Cell Physiology·Alexey M PetrovIgor I Krivoi
Mar 3, 2019·International Journal of Molecular Sciences·Igor I Krivoi, Alexey M Petrov
Mar 7, 2021·International Journal of Molecular Sciences·Irina G BryndinaAlexey M Petrov
May 20, 2020·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Guzalia F ZakyrjanovaAlexey M Petrov
Aug 16, 2021·Free Radical Biology & Medicine·Guzel F ZakyrjanovaAlexey M Petrov

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