PMID: 6989404Apr 30, 1980Paper

Effects of 5-fluorouracil on the formation of modified nucleosides in yeast transfer RNA

Biochimica Et Biophysica Acta
J D JohnsonJ Horowitz

Abstract

Yeast cells grown in the presence of the drug FUra synthesize RNA in which Urd is partially replaced by FUrd. Transfer RNAs in which 1.5-50% of the Urd has been replaced by FUrd have been isolated and their base compositions measured to determine the effect of FUrd incorporation on posttranscriptional nucleoside modification. This replacement results in an extensive reduction in the amounts of Thd, H56Urd and psird found in mature tRNA. Quantitatively, the reduction of psird greater than or equal to Thd greater than H56Urd. The losses of psird, Thd and H56Urd are greater than can be accounted for by the stoichiometry of FUrd incorporation. The formation of 5-MeCyd is not affected by the drug, whereas the methylated purines show substoichiometric losses in FUrd-containing tRNAs. In Escherichia coli, we have not observed any effects of FUra on the methylated purine content, although the effects on psird, Thd and H56Urd are similar. These findings indicate that (a) in both pro- and eukaryotic systems the enzymes responsible for psird, Thd and H56Urd formation are affected by FUra treatment in a similar manner; (b) prokaryotic purine methylases may be more tolerant of structural aberrations resulting from FUrd incorporation than eu...Continue Reading

References

Jul 10, 1979·Biochemistry·D A Frendewey, I I Kaiser
Jan 1, 1965·Progress in Nucleic Acid Research and Molecular Biology·C Heidelberger
May 1, 1968·Biochemistry·R J Lowrie, P L Bergquist
Nov 14, 1972·Journal of Molecular Biology·I I Kaiser
Jan 1, 1970·Annual Review of Biochemistry·G Attardi, F Amaldi
Oct 14, 1971·Biochimica Et Biophysica Acta·J D Johnson, J Horowitz
Dec 16, 1964·Biochimica Et Biophysica Acta·P S SARIN, P C ZAMECNIK
Dec 20, 1976·Biochemical and Biophysical Research Communications·L W LuK Randerath
May 17, 2011·The Journal of Rheumatology·Sophie VaracheAlain Saraux

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