Effects of 50 Hz electromagnetic fields on human epidermal stem cells cultured on collagen sponge scaffolds

International Journal of Radiation Biology
Wen-Fang BaiWei-Cheng Xu

Abstract

This study is to investigate the effects of electromagnetic fields (EMF) on proliferation of epidermal stem cells (ESC), which could present a viable clinical option for skin tissue engineering. The ESC obtained from human foreskin were grafted into type-I three-dimensional collagen sponge scaffolds, and then were exposed with EMF (frequency 50 Hz, intensity 5 mT) for 14 d (30 min per d). Meanwhile, the control group was set under the same conditions without EMF. The effects of EMF on growth and proliferation of ESC were analyzed with staining of hematoxylin and eosin (H&E) and 4',6-diamidino-2-phenylindole (DAPI) under microscope or scanning electron microscope. The data of DAPI staining for 2 d, 7 d, 10 d and 14 d were collected respectively to investigate the cells proliferation. ESC cultured in collagen sponge scaffolds could be steady grown and EMF could promote ESC proliferation compared with control (P < 0.05). EMF could significantly promote proliferation of ESC, which leads to a promising clinical option for skin tissue engineering.

References

Jul 1, 1983·The Journal of Investigative Dermatology·M PruniérasD Woodley
Apr 1, 1993·Journal of Cellular Biochemistry·C A Bassett
Jan 1, 1997·Bioelectromagnetics·M Blank, R Goodman
Oct 15, 1998·Experimental Cell Research·J R Bickenbach, E Chism
Apr 12, 2000·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·A J Bailey
Feb 13, 2001·Experimental Dermatology·M DunnwaldJ R Bickenbach
Mar 8, 2002·The Journal of Burn Care & Rehabilitation·A GragnaniL M Ferreira
Oct 16, 2002·Biochemical and Biophysical Research Communications·Dimitris J PanagopoulosLukas H Margaritis
Mar 15, 2003·Clinics in Plastic Surgery·Michael J Miller, Charles W Patrick
Aug 21, 2003·Bioelectromagnetics·C L M Bauréus KochJ L Eberhardt
Oct 29, 2003·Current Opinion in Biotechnology·Michel Brouard, Yann Barrandon
Jan 21, 2004·Annals of Noninvasive Electrocardiology : the Official Journal of the International Society for Holter and Noninvasive Electrocardiology, Inc·Ugur K TezcanAli Oto
Nov 19, 2004·Cellular and Molecular Life Sciences : CMLS·D-S KimK-C Park
Feb 18, 2005·Biology of the Cell·Maria I Morasso, Marjana Tomic-Canic
Jul 1, 2005·Journal of the European Academy of Dermatology and Venereology : JEADV·R Barthel, D Aberdam
Aug 24, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Teodora NikolovaAnna M Wobus
Aug 30, 2005·Archives of Biochemistry and Biophysics·A MorelliI M Pepe
Sep 2, 2006·The Journal of Hand Surgery·Berish StrauchArthur A Pilla
Mar 22, 2007·Health Care Analysis : HCA : Journal of Health Philosophy and Policy·Mairi Levitt, Neil Manson
Apr 12, 2008·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Z SchwartzB D Boyan
May 9, 2008·International Journal of Radiation Biology·X Q KeH Chiang
Mar 11, 2009·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Ming-Tzu TsaiWalter H Chang

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