Effects of 7 wk of endurance training on human skeletal muscle metabolism during submaximal exercise

Journal of Applied Physiology
Paul J LeBlancGeorge J F Heigenhauser

Abstract

This is the first study to examine the effects of endurance training on the activation state of glycogen phosphorylase (Phos) and pyruvate dehydrogenase (PDH) in human skeletal muscle during exercise. We hypothesized that 7 wk of endurance training (Tr) would result in a posttransformationally regulated decrease in flux through Phos and an attenuated activation of PDH during exercise due to alterations in key allosteric modulators of these important enzymes. Eight healthy men (22 +/- 1 yr) cycled to exhaustion at the same absolute workload (206 +/- 5 W; approximately 80% of initial maximal oxygen uptake) before and after Tr. Muscle biopsies (vastus lateralis) were obtained at rest and after 5 and 15 min of exercise. Fifteen minutes of exercise post-Tr resulted in an attenuated activation of PDH (pre-Tr: 3.75 +/- 0.48 vs. post-Tr: 2.65 +/- 0.38 mmol.min(-1).kg wet wt(-1)), possibly due in part to lower pyruvate content (pre-Tr: 0.94 +/- 0.14 vs. post-Tr: 0.46 +/- 0.03 mmol/kg dry wt). The decreased pyruvate availability during exercise post-Tr may be due to a decreased muscle glycogenolytic rate (pre-Tr: 13.22 +/- 1.01 vs. post-Tr: 7.36 +/- 1.26 mmol.min(-1).kg dry wt(-1)). Decreased glycogenolysis was likely mediated, in part, ...Continue Reading

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Citations

Nov 23, 2006·European Journal of Applied Physiology·Simon Marwood, Joanna L Bowtell
Oct 3, 2008·American Journal of Physiology. Cell Physiology·Yanjun LiMarco E Cabrera
Nov 30, 2007·Medicine and Science in Sports and Exercise·Hanneke BoonLuc J C VAN Loon
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Jun 18, 2011·Journal of Applied Physiology·Kristian KiilerichHenriette Pilegaard
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Jun 3, 2021·International Journal of Environmental Research and Public Health·Abdullah F AlghannamMaha H Alhussain

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