Effects of a ceramide-containing water-in-oil ointment on skin barrier function and allergen penetration in an IL-31 treated 3D model of the disrupted skin barrier

Experimental Dermatology
Sebastian HuthJens Malte Baron

Abstract

Atopic dermatitis (AD) is a chronically relapsing, pruritic inflammation of the skin with dryness and disturbed skin barrier function. Recently, we established that IL-31 treatment of human 3D skin models resulted in a disrupted skin barrier phenotype resembling AD. In this model, we found that IL-31 interferes with the differentiation of keratinocytes and inhibits the expression of terminal differentiation markers. In the present study, we investigated the effects of a ceramide-containing water-in-oil skin care ointment on the physical skin barrier structure and function in disrupted skin barrier models, generated either by using primary normal human epidermal keratinocytes (NHEK) or HaCaT cells. We observed that the physical skin barrier of the models recovered after daily topical treatment with the ceramide-containing ointment. Topical application of the ointment prevented downregulation of filaggrin and disorganization of other differentiation markers, such as keratin 10 and β4-integrin, as demonstrated by immunohistological analysis. The expression of Ki67 was also upregulated in response to the ointment. Furthermore, functional studies revealed that local application of the ointment diminished the increased uptake of fluo...Continue Reading

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Citations

Feb 7, 2020·Journal of Cosmetic Dermatology·Hecong WangCongfen He
Mar 13, 2020·Biogerontology·Idris Adewale AhmedAl-Shwyeh Hussah Abdullah
Mar 25, 2019·International Journal of Molecular Sciences·Min Cheol KangSun Yeou Kim
Jan 25, 2021·International Journal of Pharmaceutics·Aneta VovesnáJarmila Zbytovská
Jun 10, 2020·Endocrine, Metabolic & Immune Disorders Drug Targets·Alexandra M RascuCarmen C Diaconu
Apr 30, 2021·Annals of Dermatology·Jiyoung AhnEric Lawrence Simpson
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Irene Dini, Sonia Laneri
Oct 7, 2021·International Immunology·Hiroyuki Irie, Kenji Kabashima

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